« Previous AbstractMultibed needle trap devices for on site sampling and preconcentration of volatile breath biomarkers    Next AbstractMitogen-activated protein kinase Hog1 mediates adaptation to G1 checkpoint arrest during arsenite and hyperosmotic stress »

Anal Chem

Title:		Automated needle trap heart-cut GC/MS and needle trap comprehensive two-dimensional GC/TOF-MS for breath gas analysis in the clinical environment
Author(s):		Mieth M; Schubert JK; Groger T; Sabel B; Kischkel S; Fuchs P; Hein D; Zimmermann R; Miekisch W;
Address:		"Department of Anesthesiology and Intensive Care Medicine, University Rostock, Schillingallee 35, 18057 Rostock, Germany"
Journal Title:		Anal Chem
Year:		2010
Volume:		82
Issue:		6
Page Number:		2541 - 2551
DOI:		10.1021/ac100061k
ISSN/ISBN:		1520-6882 (Electronic) 0003-2700 (Linking)
Abstract:		"This study was intended to evaluate low-volume (20 mL) multibed needle trap (NTD) sampling combined with heart-cut gas chromatography/mass spectrometry (GC/MS) and comprehensive two-dimensional gas chromatography/time-of-flight mass spectrometry (GC x GC/TOF-MS) for trace gas analysis under clinical conditions. NTDs, high-throughput automatic desorption and separation systems, were tested in vitro and within a study in 11 patients undergoing cardiac surgery with respect to reproducibility, reliability, and clinical applicability. NTD-heart-cut GC/MS analysis of standard mixtures containing different volatile organic compounds (VOCs) yielded relative standard deviations (RSDs) from 4.0% to 18.5%. Substance adsorption was stable for 1 day if NTDs were closed on both ends and was stable for approximately 7.8 h when NTD tip ends had to be left open during autosampler storage. Even in the presence of high concentrations of contaminants linearity of heart-cut GC/MS was conserved. In patients' breath potential biomarkers could be determined even in the presence of very high concentrations of sevoflurane. Profiles of blood-borne biomarkers, intravenous drugs, and clinical contaminants were characterized. Comprehensive GC x GC/TOF-MS may be used as a screening tool for new biomarkers, if patterns are generated from deconvoluted normalized areas. Needle trap sampling in combination with hyphenated chromatographic techniques can thus be used to provide well-tailored solutions for complex problems occurring in clinical breath analysis"
Keywords:		Breath Tests/*instrumentation/methods Equipment Design Gas Chromatography-Mass Spectrometry/*methods Gases/*analysis Humans Linear Models Pulmonary Alveoli/*chemistry Reproducibility of Results;
Notes:		"MedlineMieth, Maren Schubert, Jochen K Groger, Thomas Sabel, Bastian Kischkel, Sabine Fuchs, Patricia Hein, Dietmar Zimmermann, Ralf Miekisch, Wolfram eng Comparative Study Evaluation Study 2010/02/23 Anal Chem. 2010 Mar 15; 82(6):2541-51. doi: 10.1021/ac100061k"