| Title: | HS-SPME-GC-MS Untargeted Analysis of Normal Rat Organs Ex Vivo: Differential VOC Discrimination and Fingerprint VOC Identification |
| Author(s): | Liu Y; Ge D; Zhou J; Chu Y; Zheng X; Ke L; Li P; Lu Y; Zou X; Xia L; Liu Y; Huang C; Shen C; Chu Y; |
| Address: | "Anhui Province Key Laboratory of Medical Physics and Technology, Institute of Health and Medical Technology, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei 230031, Anhui, China. University of Science and Technology of China, Hefei 230026, Anhui, China. School of Basic Medical Sciences, Anhui Medical University, Hefei 230032, Anhui, China. The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, Anhui 230001, P. R. China. Hefei Cancer Hospital, Chinese Academy of Sciences, Hefei 230031, Anhui, China" |
| DOI: | 10.1021/acs.analchem.3c01546 |
| ISSN/ISBN: | 1520-6882 (Electronic) 0003-2700 (Linking) |
| Abstract: | "The investigation of volatile organic compounds (VOCs) in human metabolites has been a topic of interest as it holds the potential for the development of non-invasive technologies to screen for organ lesions in vivo. However, it remains unclear whether VOCs differ among healthy organs. Consequently, a study was conducted to analyze VOCs in ex vivo organ tissues obtained from 16 Wistar rats, comprising 12 different organs. The VOCs released from each organ tissue were detected by the headspace-solid phase microextraction-gas chromatography-mass spectrometry technique. In the untargeted analysis of 147 chromatographic peaks, the differential volatiles of rat organs were explored based on the Mann-Whitney U test and fold change (FC > 2.0) compared with other organs. It was found that there were differential VOCs in seven organs. A discussion on the possible metabolic pathways and related biomarkers of organ differential VOCs was conducted. Based on the orthogonal partial least squares discriminant analysis and receiver operating characteristic curve, we found that differential VOCs in the liver, cecum, spleen, and kidney can be used as the unique identification of the corresponding organ. In this study, differential VOCs of organs in rats were systematically reported for the first time. Profiles of VOCs produced by healthy organs can serve as a reference or baseline that may indicate the presence of disease or abnormalities in the organ's function. Differential VOCs can be used as the fingerprint of organs, and future integration with metabolic research may contribute to the development of healthcare" |
| Notes: | "PublisherLiu, Yue Ge, Dianlong Zhou, Jijuan Chu, Yajing Zheng, Xiangxue Ke, Li Li, Pan Lu, Yan Zou, Xue Xia, Lei Liu, Yawei Huang, Chaoqun Shen, Chengyin Chu, Yannan eng 2023/07/01 21:06 Anal Chem. 2023 Aug 1; 95(30):11375-11382. doi: 10.1021/acs.analchem.3c01546. Epub 2023 Jul 1" |
