| Title: | Novel Pseudomonas sp. SCA7 Promotes Plant Growth in Two Plant Families and Induces Systemic Resistance in Arabidopsis thaliana |
| Author(s): | Kuhl-Nagel T; Rodriguez PA; Gantner I; Chowdhury SP; Schwehn P; Rosenkranz M; Weber B; Schnitzler JP; Kublik S; Schloter M; Rothballer M; Falter-Braun P; |
| Address: | "Institute for Network Biology, Helmholtz Center Munich, German Research Center for Environmental Health (GmbH), Neuherberg, Germany. Microbe-Host Interactions, Faculty of Biology, Ludwig-Maximilians-University of Munich, Munich, Germany. Institute of Biochemical Plant Pathology, Research Unit Environmental Simulation, Helmholtz Center Munich, German Research Center for Environmental Health (GmbH), Neuherberg, Germany. Research Unit for Comparative Microbiome Analysis (COMI), Helmholtz Center Munich, German Research Center for Environmental Health (GmbH), Neuherberg, Germany" |
| DOI: | 10.3389/fmicb.2022.923515 |
| ISSN/ISBN: | 1664-302X (Print) 1664-302X (Electronic) 1664-302X (Linking) |
| Abstract: | "Pseudomonas sp. SCA7, characterized in this study, was isolated from roots of the bread wheat Triticum aestivum. Sequencing and annotation of the complete SCA7 genome revealed that it represents a potential new Pseudomonas sp. with a remarkable repertoire of plant beneficial functions. In vitro and in planta experiments with the reference dicot plant A. thaliana and the original monocot host T. aestivum were conducted to identify the functional properties of SCA7. The isolate was able to colonize roots, modify root architecture, and promote growth in A. thaliana. Moreover, the isolate increased plant fresh weight in T. aestivum under unchallenged conditions. Gene expression analysis of SCA7-inoculated A. thaliana indicated a role of SCA7 in nutrient uptake and priming of plants. Moreover, confrontational assays of SCA7 with fungal and bacterial plant pathogens revealed growth restriction of the pathogens by SCA7 in direct as well as indirect contact. The latter indicated involvement of microbial volatile organic compounds (mVOCs) in this interaction. Gas chromatography-mass spectrometry (GC-MS) analyses revealed 1-undecene as the major mVOC, and octanal and 1,4-undecadiene as minor abundant compounds in the emission pattern of SCA7. Additionally, SCA7 enhanced resistance of A. thaliana against infection with the plant pathogen Pseudomonas syringae pv. tomato DC3000. In line with these results, SA- and JA/ET-related gene expression in A. thaliana during infection with Pst DC3000 was upregulated upon treatment with SCA7, indicating the ability of SCA7 to induce systemic resistance. The thorough characterization of the novel Pseudomonas sp. SCA7 showed a remarkable genomic and functional potential of plant beneficial traits, rendering it a promising candidate for application as a biocontrol or a biostimulation agent" |
| Keywords: | Arabidopsis thaliana Isr Pgpb Pseudomonas Triticum aestivum L.biocontrol mVOCs plant-microbe interactions; |
| Notes: | "PubMed-not-MEDLINEKuhl-Nagel, Theresa Rodriguez, Patricia Antonia Gantner, Isabella Chowdhury, Soumitra Paul Schwehn, Patrick Rosenkranz, Maaria Weber, Baris Schnitzler, Jorg-Peter Kublik, Susanne Schloter, Michael Rothballer, Michael Falter-Braun, Pascal eng Switzerland 2022/07/26 Front Microbiol. 2022 Jun 27; 13:923515. doi: 10.3389/fmicb.2022.923515. eCollection 2022" |
