« Previous AbstractIdentification of time-dependent biomarkers and effects of exposure to volatile organic compounds using high-throughput analysis    Next Abstract"Effects of processing procedures on the formation of aroma intensity and odor characteristic of Benshan tea (Oolong tea, Camellia sentences)" »

J Chromatogr A

Title:		Chemical profile of anti-epidemic sachet based on multiple sample preparation coupled with gas chromatography-mass spectrometry analysis combined with an embedded peaks resolution method and their action mechanisms
Author(s):		Hong L; Ye Z; Wang C; Wang J; Wang Q; Li S; Zhao J;
Address:		"Joint Laboratory of Chinese Herbal Glycoengineering and Testing Technology, University of Macau & National Glycoengineering Research Center, Macau, China; State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macau, China; Department of Pharmaceutical sciences, Faculty of Health Sciences, University of Macau, Macau, China. Electronic address: yc07543@um.edu.mo. Joint Laboratory of Chinese Herbal Glycoengineering and Testing Technology, University of Macau & National Glycoengineering Research Center, Macau, China; State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macau, China; Department of Pharmaceutical sciences, Faculty of Health Sciences, University of Macau, Macau, China. Electronic address: mc15369@um.edu.mo. Joint Laboratory of Chinese Herbal Glycoengineering and Testing Technology, University of Macau & National Glycoengineering Research Center, Macau, China; State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macau, China; Department of Pharmaceutical sciences, Faculty of Health Sciences, University of Macau, Macau, China. Electronic address: yc07545@um.edu.mo. National Institute of TCM constitution and Prevention Medicine, Beijing University of Chinese Medicine, Beijing, China. National Institute of TCM constitution and Prevention Medicine, Beijing University of Chinese Medicine, Beijing, China. Electronic address: wangqi710@126.com. Joint Laboratory of Chinese Herbal Glycoengineering and Testing Technology, University of Macau & National Glycoengineering Research Center, Macau, China; State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macau, China; Department of Pharmaceutical sciences, Faculty of Health Sciences, University of Macau, Macau, China. Electronic address: spli@um.edu.mo. Joint Laboratory of Chinese Herbal Glycoengineering and Testing Technology, University of Macau & National Glycoengineering Research Center, Macau, China; State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macau, China; Department of Pharmaceutical sciences, Faculty of Health Sciences, University of Macau, Macau, China. Electronic address: jingzhao@um.edu.mo"
Journal Title:		J Chromatogr A
Year:		2023
Volume:		20230121
Issue:
Page Number:		463816 -
DOI:		10.1016/j.chroma.2023.463816
ISSN/ISBN:		1873-3778 (Electronic) 0021-9673 (Linking)
Abstract:		"The anti-epidemic sachet (Fang Yi Xiang Nang, FYXN) in traditional Chinese medicine (TCM) can prevent COVID-19 through volatile compounds that can play the role of fragrant and dampness, heat-clearing and detoxifying, warding off filth and pathogenic factors. Nevertheless, the anti-(mutant) SARS-CoV-2 compounds and the compounds related to the mechanism in vivo, and the mechanism of FYXN are still vague. In this study, the volatile compound set of FYXN was constructed by gas chromatography-mass spectrometry (GC-MS) based on multiple sample preparation methods, which include headspace (HS), headspace solid phase microextraction (HS-SPME) and pressurized liquid extraction (PLE). In addition, selective ion analysis (SIA) was used to resolve embedded chromatographic peaks present in HS-SPME results. Preliminary analysis of active compounds and mechanism of FYXN by network pharmacology combined with disease pathway information based on GC-MS results. A total of 96 volatile compounds in FYXN were collected by GC-MS analysis. 39 potential anti-viral compounds were screened by molecular docking. 13 key pathways were obtained by KEGG pathway analysis (PI3K-Akt signaling pathway, HIF-1 signaling pathway, etc.) for FYXN to prevent COVID-19. 16 anti-viral compounds (C95, C91, etc.), 10 core targets (RELA, MAPK1, etc.), and 16 key compounds related to the mechanism in vivo (C56, C30, etc.) were obtained by network analysis. The relevant pharmacological effects of key pathways and key compounds were verified by the literature. Finally, molecular docking was used to verify the relationship between core targets and key compounds, which are related to the mechanism in vivo. A variety of sample preparation methods coupled with GC-MS analysis combined with an embedded peaks resolution method and integrated with network pharmacology can not only comprehensively characterize the volatile compounds in FYXN, but also expand the network pharmacology research ideas, and help to discover the active compounds and mechanisms in FYXN"
Keywords:		Humans Gas Chromatography-Mass Spectrometry/methods Molecular Docking Simulation Phosphatidylinositol 3-Kinases *covid-19 SARS-CoV-2 Solid Phase Microextraction/methods *Volatile Organic Compounds/analysis Anti-epidemic sachet Covid-19 Chemometrics Gas ch;
Notes:		"MedlineHong, Liang Ye, Zihui Wang, Cheng Wang, Ji Wang, Qi Li, Shaoping Zhao, Jing eng Netherlands 2023/01/31 J Chromatogr A. 2023 Feb 22; 1691:463816. doi: 10.1016/j.chroma.2023.463816. Epub 2023 Jan 21"