| Title: | Bioorganic research of Galactites tomentosa Moench. Honey extracts: enantiomeric purity of chiral marker 3-phenyllactic acid |
| Author(s): | Jerkovic I; Roje M; Tuberoso CI; Marijanovic Z; Kasum A; Obradovic M; |
| Address: | "Department of Organic Chemistry, Faculty of Chemistry & Technology, University of Split, Split, Croatia" |
| ISSN/ISBN: | 1520-636X (Electronic) 0899-0042 (Linking) |
| Abstract: | "Thistle (Galactites tomentosa Moench.) honey organic extracts were obtained by headspace solid-phase microextraction (HS-SPME) and ultrasonic solvent extraction (USE) and analyzed by gas chromatography (GC-FID and GC-MS) for the first time. Most abundant headspace compounds were terpenes, particularly linalool derivatives (hotrienol was predominant with a range of 38.6-57.5%). 3-Phenyllactic acid dominated in the solvent extracts (77.4-86.4%) followed by minor percentages of other shikimate pathway derivatives. After determination of an adequate enantioseparation protocol on Chirallica PST-4 column, the honey solvent extracts were analyzed by high-performance liquid chromatography (HPLC). The chiral analysis revealed high enantiomeric excess (>95%) of (-)-3-phenyllactic acid in all samples. Therefore, previous findings of chemical markers of thistle honey were extended, providing new potential for advanced chemical fingerprinting (optical pure chemical marker)" |
| Keywords: | Asteraceae/*chemistry Gas Chromatography-Mass Spectrometry *Honey Lactates/*chemistry/*isolation & purification Solid Phase Microextraction Solvents/chemistry Stereoisomerism Ultrasonics Volatilization (-)-3-phenyllactic acid Gc-fid gc-ms chiral HPLC with; |
| Notes: | "MedlineJerkovic, Igor Roje, Marin Tuberoso, Carlo I G Marijanovic, Zvonimir Kasum, Ana Obradovic, Marina eng Research Support, Non-U.S. Gov't 2014/05/23 Chirality. 2014 Aug; 26(8):405-10. doi: 10.1002/chir.22340. Epub 2014 May 22" |
