| Title: | A Wheat beta-Patchoulene Synthase Confers Resistance Against Herbivory in Transgenic Arabidopsis |
| Author(s): | Pu Q; Liang J; Shen Q; Fu J; Pu Z; Liu J; Wang X; Wang Q; |
| Address: | "Institute of Ecological Agriculture, College of Agronomy, Sichuan Agricultural University, Chengdu 611130, China. cnpuqingyu@sina.com. Institute of Ecological Agriculture, College of Agronomy, Sichuan Agricultural University, Chengdu 611130, China. L_Jinch@126.com. Institute of Ecological Agriculture, College of Agronomy, Sichuan Agricultural University, Chengdu 611130, China. shenqin2000@sina.cn. Institute of Ecological Agriculture, College of Agronomy, Sichuan Agricultural University, Chengdu 611130, China. fjy0204@sina.com. Institute of Ecological Agriculture, College of Agronomy, Sichuan Agricultural University, Chengdu 611130, China. puzhien@sicau.edu.cn. Institute of Ecological Agriculture, College of Agronomy, Sichuan Agricultural University, Chengdu 611130, China. jiangliu@sicau.edu.cn. Institute of Ecological Agriculture, College of Agronomy, Sichuan Agricultural University, Chengdu 611130, China. wangxuegui@sicau.edu.cn" |
| ISSN/ISBN: | 2073-4425 (Print) 2073-4425 (Electronic) 2073-4425 (Linking) |
| Abstract: | "Terpenoids play important roles in plant defense. Although some terpene synthases have been characterized, terpenoids and their biosynthesis in wheat (Triticumaestivum L.) still remain largely unknown. Here, we describe the identification of a terpene synthase gene in wheat. It encodes a sesquiterpene synthase that catalyzes beta-patchoulene formation with E,E-farnesyl diphosphate (FPP) as the substrate, thus named as TaPS. TaPS exhibits inducible expression in wheat in response to various elicitations. Particularly, alamethicin treatment strongly induces TaPS gene expression and beta-patchoulene accumulation in wheat. Overexpression of TaPS in Arabidopsis successfully produces beta-patchoulene, verifying the biochemical function of TaPS in planta. Furthermore, these transgenic Arabidopsis plants exhibit resistance against herbivory by repelling beet armyworm larvae feeding, thereby indicating anti-herbivory activity of beta-patchoulene. The catalytic mechanism of TaPS is also explored by homology modeling and site-directed mutagenesis. Two key amino acids are identified to act in protonation and stability of intermediates and product formation. Taken together, one wheat sesquiterpene synthase is identified as beta-patchoulene synthase. TaPS exhibits inducible gene expression and the sesquiterpene beta-patchoulene is involved in repelling insect infestation" |
| Keywords: | "Alkyl and Aryl Transferases/chemistry/*genetics/pharmacology Animals Arabidopsis/chemistry/*genetics Gene Expression Regulation, Plant/genetics Helminths/drug effects/pathogenicity Herbivory/drug effects/genetics Plants, Genetically Modified/*genetics/gro;" |
| Notes: | "MedlinePu, Qingyu Liang, Jin Shen, Qinqin Fu, Jingye Pu, Zhien Liu, Jiang Wang, Xuegui Wang, Qiang eng Research Support, Non-U.S. Gov't Switzerland 2019/06/13 Genes (Basel). 2019 Jun 10; 10(6):441. doi: 10.3390/genes10060441" |
