| Title: | Profiling volatile compounds from culture supernatants of periodontal bacteria using gas chromatography/mass spectrometry/olfactometry analysis with a monolithic silica gel adsorption device |
| Author(s): | Mori A; Taniguchi M; Kuboniwa M; Amano A; Fukusaki E; |
| Address: | "Department of Biotechnology, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan. Osaka University Shimadzu Omics Innovation Research Laboratories, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan. Department of Preventive Dentistry, Graduate School of Dentistry, Osaka University, 1-8 Yamadaoka, Suita, Osaka 565-0871, Japan. Department of Biotechnology, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan; Osaka University Shimadzu Omics Innovation Research Laboratories, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan; Industrial Biotechnology Initiative Division, Institute for Open and Transdisciplinary Research Initiative, Osaka University, 1-1 Yamadaoka, Suita, Osaka 565-0871, Japan. Electronic address: fukusaki@bio.eng.osaka-u.ac.jp" |
| DOI: | 10.1016/j.jbiosc.2022.04.002 |
| ISSN/ISBN: | 1347-4421 (Electronic) 1347-4421 (Linking) |
| Abstract: | "Halitosis is formed mainly by the volatile compounds produced by periodontal bacteria. Three volatile sulfur compounds (VSCs), hydrogen sulfide, methanethiol, and dimethyl sulfide, have attracted attention as major components of halitosis. However, these compounds cannot account for all odors. In this study, we profiled volatile compounds from the culture supernatants of periodontal bacteria using gas chromatography/mass spectrometry/olfactometry analysis with a monolithic silica gel adsorption device to investigate the potential odorous compounds. Periodontal bacteria have been found to produce volatile compounds belonging to various classes, such as alcohols, ketones, fatty acids, and aromatic compounds, in addition to VSCs. In addition, VSCs different from hydrogen sulfide and methanethiol, which are considered important causative compounds, may also influence to halitosis" |
| Keywords: | Adsorption Bacteria Gas Chromatography-Mass Spectrometry *Halitosis/microbiology Humans *Hydrogen Sulfide/analysis Odorants/analysis Olfactometry Silica Gel Sulfur Compounds/analysis *Volatile Organic Compounds/analysis Gas chromatography/mass spectrometr; |
| Notes: | "MedlineMori, Asuka Taniguchi, Moyu Kuboniwa, Masae Amano, Atsuo Fukusaki, Eiichiro eng Japan 2022/04/29 J Biosci Bioeng. 2022 Jul; 134(1):77-83. doi: 10.1016/j.jbiosc.2022.04.002. Epub 2022 Apr 26" |
