| Title: | "Release of free and conjugated forms of the putative pheromonal steroid 11-oxo-etiocholanolone by reproductively mature male round goby (Neogobius melanostomus Pallas, 1814)" |
| Author(s): | Katare YK; Scott AP; Laframboise AJ; Li W; Alyasha'e Z; Caputo CB; Loeb SJ; Zielinski B; |
| Address: | "Department of Biological Sciences, University of Windsor, Windsor, Ontario, Canada" |
| DOI: | 10.1095/biolreprod.110.086546 |
| ISSN/ISBN: | 1529-7268 (Electronic) 0006-3363 (Linking) |
| Abstract: | "Previous studies of the round goby (Neogobius melanostomus Pallas, 1814), an invasive fish species in the Laurentian Great Lakes of North America, have shown that this species has the ability to both synthesize and smell steroids that have a 5 beta-reduced and 3 alpha-hydroxyl (5 beta,3 alpha) configuration. An enzyme-linked immunoassay (EIA) for 3 alpha-hydroxy-5 beta-androstane-11,17-dione (11-O-ETIO) has been used to show a substantial rise in the rate of release of immunoreactive compounds into the water when males are injected with salmon gonadotropin releasing hormone analogue. Similar increases were noted for 11-ketotestosterone and 17,20 beta-dihydroxypregn-4-en-3-one. Partitioning of the extracts between diethyl ether and water showed the presence of both free and conjugated immunoreactive 11-O-ETIO. Only conjugated immunoreactivity was found in urine (implying that free steroid is released via the gills). The identity of the conjugates was probed by using HPLC, EIA, and mass spectrometry and removal of sulfate and glucosiduronate groups. Immunoreactivity in the conjugated fraction was found to be due mainly to 3 alpha,17beta-dihydroxy-5 beta-androstan-11-one 17-sulfate. However, the evidence was also strong for the presence in water extracts of substantial amounts of 3 alpha-hydroxy-5 beta-androstane-11,17-dione 3-glucosiduronate (which could be detected only by EIA after removal of the glucosiduronate group with beta-glucuronidase). There were also small amounts of 3 alpha-hydroxy-5 beta-androstane-11,17-dione 3-sulfate and 3 alpha,17beta-dihydroxy-5 beta-androstan-11-one 17-glucosiduronate. These studies give some idea of the types, amounts, and ratios of 11-O-ETIO derivatives that are released by reproductive N. melanostomus and will aid further research into the putative pheromonal roles of 5 beta,3 alpha-reduced androgens in this species" |
| Keywords: | "Animals Chemical Fractionation/methods Chromatography, High Pressure Liquid Etiocholanolone/*analogs & derivatives/analysis/chemistry Gonadotropin-Releasing Hormone/administration & dosage/analogs & derivatives Hydroxyprogesterones/analysis Immunoenzyme T;" |
| Notes: | "MedlineKatare, Yogesh Kumar Scott, Alexander P Laframboise, Alyson J Li, Weiming Alyasha'e, Zena Caputo, Christopher B Loeb, Stephen J Zielinski, Barbara eng Research Support, Non-U.S. Gov't 2010/10/15 Biol Reprod. 2011 Feb; 84(2):288-98. doi: 10.1095/biolreprod.110.086546. Epub 2010 Oct 13" |
