| Title: | A pleiotropic drug resistance transporter in Nicotiana tabacum is involved in defense against the herbivore Manduca sexta |
| Author(s): | Bienert MD; Siegmund SE; Drozak A; Trombik T; Bultreys A; Baldwin IT; Boutry M; |
| Address: | "Institut des Sciences de la Vie, Universite Catholique de Louvain, Croix du Sud 4-15, 1348 Louvain la Neuve, Belgium" |
| DOI: | 10.1111/j.1365-313X.2012.05108.x |
| ISSN/ISBN: | 1365-313X (Electronic) 0960-7412 (Linking) |
| Abstract: | "Pleiotropic drug resistance (PDR) transporters are a group of membrane proteins belonging to the ABCG sub-family of ATP binding cassette (ABC) transporters. There is clear evidence for the involvement of plant ABC transporters in resistance to fungal and bacterial pathogens, but not in the biotic stress response to insect or herbivore attack. Here, we describe a PDR transporter, ABCG5/PDR5, from Nicotiana tabacum. GFP fusion and subcellular fractionation studies revealed that ABCG5/PDR5 is localized to the plasma membrane. Staining of transgenic plants expressing the GUS reporter gene under the control of the ABCG5/PDR5 transcription promoter and immunoblotting of wild-type plants showed that, under standard growth conditions, ABCG5/PDR5 is highly expressed in roots, stems and flowers, but is only expressed at marginal levels in leaves. Interestingly, ABCG5/PDR5 expression is induced in leaves by methyl jasmonate, wounding, pathogen infiltration, or herbivory by Manduca sexta. To address the physiological role of ABCG5/PDR5, N. tabacum plants silenced for the expression of ABCG5/PDR5 were obtained. No phenotypic modification was observed under standard conditions. However, a small increase in susceptibility to the fungus Fusarium oxysporum was observed. A stronger effect was observed in relation to herbivory: silenced plants allowed better growth and faster development of M. sexta larvae than wild-type plants, indicating an involvement of this PDR transporter in resistance to M. sexta herbivory" |
| Keywords: | "Acetates/metabolism/pharmacology Animals Base Sequence Cell Membrane/metabolism Cloning, Molecular Cyclopentanes/metabolism/pharmacology Flowers/genetics Fusarium/pathogenicity Gene Expression Regulation, Plant Gene Silencing Green Fluorescent Proteins/ge;" |
| Notes: | "MedlineBienert, Manuela D Siegmund, Stephanie E G Drozak, Anna Trombik, Tomasz Bultreys, Alain Baldwin, Ian T Boutry, Marc eng Research Support, Non-U.S. Gov't England 2012/07/19 Plant J. 2012 Dec; 72(5):745-57. doi: 10.1111/j.1365-313X.2012.05108.x. Epub 2012 Oct 15" |
