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G3 (Bethesda)

Title:		"Transcriptome analysis implicates secondary metabolite production, redox reactions, and programmed cell death during allorecognition in Cryphonectria parasitica"
Author(s):		Belov AA; Witte TE; Overy DP; Smith ML;
Address:		"Department of Biology, Carleton University, Ottawa, ON K1S 5B6, Canada. Agriculture and Agri-Food Canada, Ottawa, ON, K1Y 4X2, Canada"
Journal Title:		G3 (Bethesda)
Year:		2021
Volume:		11
Issue:		1
Page Number:		-
DOI:		10.1093/g3journal/jkaa021
ISSN/ISBN:		2160-1836 (Electronic) 2160-1836 (Linking)
Abstract:		"The underlying molecular mechanisms of programmed cell death associated with fungal allorecognition, a form of innate immunity, remain largely unknown. In this study, transcriptome analysis was used to infer mechanisms activated during barrage formation in vic3-incompatible strains of Cryphonectria parasitica, the chestnut blight fungus. Pronounced differential expression occurred in barraging strains of genes involved in mating pheromone (mf2-1, mf2-2), secondary metabolite production, detoxification (including oxidative stress), apoptosis-related, RNA interference, and HET-domain genes. Evidence for secondary metabolite production and reactive oxygen species (ROS) accumulation is supported through UPLC-HRMS analysis and cytological staining, respectively. Differential expression of mating-related genes and HET-domain genes was further examined by RT-qPCR of incompatible interactions involving each of the six vegetative incompatibility (vic) loci in C. parasitica and revealed distinct recognition process networks. We infer that vegetative incompatibility in C. parasitica activates defence reactions that involve secondary metabolism, resulting in increased toxicity of the extra- and intracellular environment. Accumulation of ROS (and other potential toxins) may result in detoxification failure and activation of apoptosis, sporulation, and the expression of associated pheromone genes. The incompatible reaction leaves abundant traces of a process-specific metabolome as conidiation is initiated"
Keywords:		*Apoptosis Ascomycota *Gene Expression Profiling Oxidation-Reduction Plant Diseases RNA-seq allorecognition heterokaryon incompatibility programmed cell death secondary metabolites transcriptomics;
Notes:		"MedlineBelov, Anatoly A Witte, Thomas E Overy, David P Smith, Myron L eng Research Support, Non-U.S. Gov't England 2021/02/10 G3 (Bethesda). 2021 Jan 18; 11(1):jkaa021. doi: 10.1093/g3journal/jkaa021"