| Title: | Sex Determination in Ceratopteris richardii Is Accompanied by Transcriptome Changes That Drive Epigenetic Reprogramming of the Young Gametophyte |
| Author(s): | Atallah NM; Vitek O; Gaiti F; Tanurdzic M; Banks JA; |
| Address: | "Purdue University Center for Cancer Research. College of Computer and Information Science, Northeastern University, Boston, MA 02115. Department of Medicine, Weill Cornell Medicine and New York Genome Center, New York, NY 10013. School of Biological Sciences, The University of Queensland, St. Lucia, Australia banksj@purdue.edu m.tanurdzic@uq.edu.au. Department of Botany and Plant Pathology, Purdue University, West Lafayette, IN 47907 banksj@purdue.edu m.tanurdzic@uq.edu.au" |
| ISSN/ISBN: | 2160-1836 (Electronic) 2160-1836 (Linking) |
| Abstract: | "The fern Ceratopteris richardii is an important model for studies of sex determination and gamete differentiation in homosporous plants. Here we use RNA-seq to de novo assemble a transcriptome and identify genes differentially expressed in young gametophytes as their sex is determined by the presence or absence of the male-inducing pheromone called antheridiogen. Of the 1,163 consensus differentially expressed genes identified, the vast majority (1,030) are up-regulated in gametophytes treated with antheridiogen. GO term enrichment analyses of these DEGs reveals that a large number of genes involved in epigenetic reprogramming of the gametophyte genome are up-regulated by the pheromone. Additional hormone response and development genes are also up-regulated by the pheromone. This C. richardii gametophyte transcriptome and gene expression dataset will prove useful for studies focusing on sex determination and differentiation in plants" |
| Keywords: | "Computational Biology/methods *Epigenesis, Genetic Ferns/*genetics Gene Expression Profiling Gene Expression Regulation, Plant Gene Ontology Gene Regulatory Networks *Genes, Plant *Germ Cells, Plant Molecular Sequence Annotation *Sex Determination Process;" |
| Notes: | "MedlineAtallah, Nadia M Vitek, Olga Gaiti, Federico Tanurdzic, Milos Banks, Jo Ann eng Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. England 2018/05/04 G3 (Bethesda). 2018 Jul 2; 8(7):2205-2214. doi: 10.1534/g3.118.200292" |
