| Title: | Identification and expression profiling of pheromone biosynthesis activating neuropeptide in Chlumetia transversa (Walker) |
| Author(s): | Wei H; Chang H; Zheng L; Lin S; Chen Y; Tian H; Zhao J; Chen Y; Cai H; Gu X; Murugan K; |
| Address: | "Institute of Plant Protection, ', 247 Wusi Road, Fuzhou 350003, China; Fujian Key Laboratory for Monitoring and Integrated Management of Crop Pests, 247 Wusi Road, Fuzhou 350003, China. Electronic address: weihui@faas.cn. Institute of Plant Protection, ', 247 Wusi Road, Fuzhou 350003, China; College of Plant Protection, Fujian Agriculture and Forestry University, 15 Shangxia Dian Road, Fuzhou 350002, China. Institute of Plant Protection, ', 247 Wusi Road, Fuzhou 350003, China; Fujian Key Laboratory for Monitoring and Integrated Management of Crop Pests, 247 Wusi Road, Fuzhou 350003, China. Fishery college, Jimei University, 43 Yindou Road, Xiamen 361021, China. College of Plant Protection, Fujian Agriculture and Forestry University, 15 Shangxia Dian Road, Fuzhou 350002, China. Electronic address: guxiaojun1@163.com. Division of Entomology, Department of Zoology, School of Life Sciences, Bharathiar University, Coimbatore 641 046, Tamil Nadu, India" |
| DOI: | 10.1016/j.pestbp.2016.05.005 |
| ISSN/ISBN: | 1095-9939 (Electronic) 0048-3575 (Linking) |
| Abstract: | "Insect neuropeptides (NPs) in the pyrokinin/pheromone biosynthesis-activating neuropeptide (PBAN) family are actively involved in many essential endocrine functions. These peptides are potential targets in the search for novel insect control agents. This is the first report on the cloning and sequence determination of Chlumetia transversa (Walker) PBAN (Ct-PBAN) using rapid amplification of cDNA ends. The open reading frame of Ct-PBAN was 588bp in length and encoded 195 amino acids, which were assembled into five putative neuropeptides (diapause hormone homolog, alpha-neuropeptide, beta-neuropeptide, PBAN, and gamma-neuropeptide). These peptides were amidated at C-terminus and shared the conserved pentapeptide motif FXPR (or K) L. Moreover, Ct-PBAN had high homology to PBANs in Helicoverpa zea (84.1%), Helicoverpa armigera (83.5%), Helicoverpa assulta (83%), and Heliothis virescens (82.6%). Phylogenetic analysis showed that Ct-PBAN was closely related to its orthologs in the family Noctuidae. In addition, real-time quantitative polymerase chain reaction assays showed that the expression of Ct-PBAN peaked in the female head and was also detected at high levels in 1-d-old adults. These results suggested that Ct-PBAN is associated with sex pheromone biosynthesis in female C. transversa and could be used for developing C. transversa control systems based on molecular techniques" |
| Keywords: | "Amino Acid Sequence Animals Base Sequence Cloning, Molecular DNA, Complementary/genetics Female Gene Expression Profiling Insect Proteins/*genetics Male Moths/*genetics Neuropeptides/*genetics Phylogeny Sequence Analysis, DNA Diapause hormone Endocrine re;" |
| Notes: | "MedlineWei, Hui Chang, Hong Zheng, Lizhen Lin, Shuo Chen, Yixin Tian, Houjun Zhao, Jianwei Chen, Yong Cai, Hongjiao Gu, Xiaojun Murugan, Kadarkarai eng 2017/01/04 Pestic Biochem Physiol. 2017 Jan; 135:89-96. doi: 10.1016/j.pestbp.2016.05.005. Epub 2016 May 31" |
