| Title: | Cellular and transcriptional responses of yeast to the cleavage of cytosolic tRNAs induced by colicin D |
| Author(s): | Shigematsu M; Ogawa T; Kido A; Kitamoto HK; Hidaka M; Masaki H; |
| Address: | "Department of Biotechnology, University of Tokyo, Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan" |
| ISSN/ISBN: | 1097-0061 (Electronic) 0749-503X (Linking) |
| Abstract: | "Colicin D is a plasmid-encoded antibacterial protein that specifically cleaves the anticodon loops of four Escherichia coli tRNA(Arg) species. Here, we report that the catalytic domain of colicin D, which is expressed in Saccharomyces cerevisiae, impairs cell growth by cleaving specific tRNAs. DNA microarray analysis revealed that mating-related genes were upregulated, while genes involved in a range of metabolic processes were downregulated, thereby impairing cell growth. The pheromone-signalling pathway was activated only in alpha cells by tRNA cleavage, which was not observed in 'a' cells or diploid cells. On the basis of these results and on the recent identification of two killer toxins that cleave specific tRNAs, the relationship between tRNA depletion and the resultant cellular response is discussed" |
| Keywords: | Base Sequence Cell Proliferation Cytosol/metabolism Escherichia coli Proteins/chemistry/genetics/*metabolism Nucleic Acid Conformation Oligonucleotide Array Sequence Analysis Peptide Fragments/chemistry/genetics/metabolism Pheromones/metabolism Plasmids/g; |
| Notes: | "MedlineShigematsu, Megumi Ogawa, Tetsuhiro Kido, Atsuhiro Kitamoto, Hiroko K Hidaka, Makoto Masaki, Haruhiko eng Research Support, Non-U.S. Gov't England 2009/10/31 Yeast. 2009 Dec; 26(12):663-73. doi: 10.1002/yea.1725" |
