| Title: | Expression levels of transdominant peptides and proteins in Saccharomyces cerevisiae |
| Author(s): | Sandrock T; Poritz M; Kim M; Feldhaus MJ; Roth B; Caponigro G; Kamb A; |
| Address: | "Deltagen Proteomics, Inc., 615 Arapeen Drive, Suite 300, Salt Lake City, UT 84108, USA. Tsandrock@arcanis.com" |
| ISSN/ISBN: | 0749-503X (Print) 0749-503X (Linking) |
| Abstract: | "From libraries of peptides and protein fragments, several inhibitors that block pheromone response in Saccharomyces cerevisiae have been isolated previously. In many cases, the inhibitors are displayed as part of a scaffold, such as green fluorescent protein. Each of the inhibitors has a characteristic physiological strength or genetic penetrance. In this report, the roles of expression level and display scaffold on the activities of a subset of pheromone-response pathway inhibitors were examined. Special consideration was given to the relationship between expression levels of specific inhibitors, which may exceed 50 microM in some instances, and penetrance" |
| Keywords: | "Adaptor Proteins, Vesicular Transport Fungal Proteins/analysis/*metabolism Gene Expression Green Fluorescent Proteins Luminescent Proteins/metabolism Membrane Proteins/analysis/metabolism Peptides/analysis/metabolism Pheromones/*antagonists & inhibitors S;" |
| Notes: | "MedlineSandrock, Tanya Poritz, Mark Kim, Marianne Feldhaus, Michael J Roth, Bruce Caponigro, Giordano Kamb, Alexander eng England 2002/01/05 Yeast. 2002 Jan 15; 19(1):1-7. doi: 10.1002/yea.795" |
