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Antonie Van Leeuwenhoek

Title:		Novel metabolites from Bacillus safensis and their antifungal property against Alternaria alternata
Author(s):		Prakash J; Arora NK;
Address:		"Department of Environmental Microbiology, School of Earth and Environmental Sciences, Babasaheb Bhimrao Ambedkar University, Lucknow, India. Department of Environmental Science, School of Earth and Environmental Sciences, Babasaheb Bhimrao Ambedkar University, Vidya Vihar, Raebareli Road, Lucknow, Uttar Pradesh, 226 025, India. nkarora_net@rediffmail.com"
Journal Title:		Antonie Van Leeuwenhoek
Year:		2021
Volume:		20210602
Issue:		8
Page Number:		1245 - 1258
DOI:		10.1007/s10482-021-01598-4
ISSN/ISBN:		1572-9699 (Electronic) 0003-6072 (Linking)
Abstract:		"Plant growth promoting rhizobacteria offer an effective and eco-sustainable solution to protect crops against phytopathogens. In the present study, Bacillus safensis STJP (NAIMCC-B-02323) from the rhizospheric soil of Stevia rebaudiana showed strong biocontrol activity against phytopathogen, Alternaria alternata. B. safensis STJP produced antifungal volatile organic compounds (AVOC). In the presence of AVOC, there was no conidia germination, mycelium growth was inhibited, and hyphae ruptured as observed by scanning electron microscopy. When mycelium of the fungus from bacterial treated plate was transferred into fresh potato dextrose agar plate, A. alternata could not grow. Extracted AVOC from B. safensis STJP were identified by thin-layer chromatography (TLC), Fourier-transform-infrared (FTIR) spectroscopy and gas-chromatography-mass spectrometry (GC-MS). In total 25 bacterial metabolites were identified by GC-MS analysis having alcohol, alkane, phenol, alkyl halide and aromatic compounds. Five of these (phenol, 2,4-bis (1,1-dimethylethyl)-, 3-hexadecanol, pyrrolo(1,2-a)pyrazine-1,4-dione, hexahydro-3-(2-methylpropyl)-, 5,10-diethoxy-2,3,7,8-tetrahydro-1H,6H-dipyrrolo(1,2-a:1',2'-d)pyrazine and hexadecanoic acid) inhibited the mycelium growth, controlling spore formation and conidia germination of A. alternata. This study concluded that AVOC producing B. safensis can be used as a green-fungicide against A. alternata. Bacterial metabolites could pave the way for the development of next generation biopesticides. This can be a reliable technology to enhance the quality and reliability of biopesticides"
Keywords:		*Alternaria *Antifungal Agents/pharmacology Bacillus Reproducibility of Results Alternaria alternata Antifungal organic volatile compounds Bacillus safensis Biocontrol Metabolites;
Notes:		"MedlinePrakash, Jai Arora, Naveen Kumar eng Netherlands 2021/06/03 Antonie Van Leeuwenhoek. 2021 Aug; 114(8):1245-1258. doi: 10.1007/s10482-021-01598-4. Epub 2021 Jun 2"