| Title: | The effect of a high-roughage diet on the metabolism of aromatic compounds by rumen microbes: a metagenomic study using Mehsani buffalo (Bubalus bubalis) |
| Author(s): | Prajapati VS; Purohit HJ; Raje DV; Parmar N; Patel AB; Jones OAH; Joshi CG; |
| Address: | "Department of Animal Biotechnology, College of Veterinary Science and Animal Husbandry, Anand Agricultural University (AAU), Anand, Gujarat, India. Environmental Genomics Division, National Environmental Engineering Research Institute (CSIR-NEERI), Nagpur, Maharashtra, India. MDS Bio-Analytics Pvt. Ltd., Nagpur, Maharashtra, India. Australian Centre for Research on Separation Science (ACROSS), School of Applied Sciences, RMIT University, GPO Box 2476, Melbourne, VIC, 3001, Australia. Department of Animal Biotechnology, College of Veterinary Science and Animal Husbandry, Anand Agricultural University (AAU), Anand, Gujarat, India. cgjoshi@aau.in" |
| Journal Title: | Appl Microbiol Biotechnol |
| DOI: | 10.1007/s00253-015-7239-0 |
| ISSN/ISBN: | 1432-0614 (Electronic) 0175-7598 (Linking) |
| Abstract: | "In developing countries, livestock are often fed a high-lignin, low-nutrient diet that is rich in aromatic compounds. It is therefore important to understand the structure of the microbial community responsible for the metabolism of these substances. A metagenomic analysis was therefore carried out to assess the microbial communities associated with the liquid and solid fractions of rumen biomaterial from domestic Mehsani buffalo (Bubalus bubalis) fed with varying proportions of roughage. The experimental design consisted of three feeding regimes (50, 75 and 100 % roughage) and two roughage types (green and dry). Genes associated with aromatic compound degradation were assessed via high-throughput DNA sequencing. A total of 3914.94 Mb data were generated from all treatment groups. Genes coding for functional responses associated with aromatic compound metabolism were more prevalent in the liquid fraction of rumen samples than solid fractions. Statistically significant differences (p < 0.05) were also observed between treatment groups. These differences were dependent on the proportion of roughage fed to the animal, with the type of roughage having little effect. The genes present in the highest abundance in all treatment groups were those related to aromatic compound catabolism. At the phylum level, Bacteroidetes were dominant in all treatments closely followed by the Firmicutes. This study demonstrates the use of feed type to selectively enrich microbial communities capable of metabolizing aromatic compounds in the rumen of domestic buffalo. The results may help to improve nutrient utilization efficiency in livestock and are thus of interest to farming industries, particularly in developing countries, worldwide" |
| Keywords: | Animal Feed/*analysis Animals Bacteria/classification/genetics/isolation & purification/*metabolism Biodiversity Buffaloes/metabolism/*microbiology Dietary Fiber/*metabolism *Gastrointestinal Microbiome Metagenomics Phylogeny Rumen/metabolism/*microbiolog; |
| Notes: | "MedlinePrajapati, Vimalkumar S Purohit, Hemant J Raje, Dhananjay V Parmar, Nidhi Patel, Anand B Jones, Oliver A H Joshi, Chaitanya G eng Research Support, Non-U.S. Gov't Germany 2015/12/30 Appl Microbiol Biotechnol. 2016 Feb; 100(3):1319-1331. doi: 10.1007/s00253-015-7239-0. Epub 2015 Dec 28" |
