| Title: | Characterization and expression pattern analysis of pheromone receptor-like genes in Winter Mushroom Flammulina filiformis |
| Author(s): | Meng L; Chou T; Jiang S; Wang L; Zhu M; Mukhtar I; Xie B; Wang W; |
| Address: | "Shandong Provincial Key Laboratory of Agricultural Microbiology, College of Plant Protection, Shandong Agricultural University, Tai'an, 271018, China. Mycological Research Center, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, 350002, China. BGI Education Center, University of Chinese Academy of Sciences, Shenzhen, 518083, China. Shandong Provincial Key Laboratory of Agricultural Microbiology, College of Plant Protection, Shandong Agricultural University, Tai'an, 271018, China. 524217669@qq.com. Mycological Research Center, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, 350002, China. 524217669@qq.com" |
| DOI: | 10.1007/s00203-020-01990-0 |
| ISSN/ISBN: | 1432-072X (Electronic) 0302-8933 (Linking) |
| Abstract: | "Pheromone receptor-like genes (PRLGs) belong to the G protein-coupled receptors (GPCRs) family that interacts with biotic and abiotic stimulants and transmits signals to intracellular downstream pathways in eukaryotic cells. In this study, we investigated the structure and expressions patterns of PRLGs in Winter Mushroom Flammulina filiformis. Based on the alignment analysis, the structure of PRLGs was found conserved in F. filiformis strains expect few single-nucleotide polymorphism (SNP) sites. Six PRLGs were found at five different unlinked loci, scattered in the genomes of F. filiformis strains. These genes contain 2-5 introns; however, the introns were not found in the same relative positions regarding the encoded protein sequences in tested strains of F. filiformis. Three conserved motifs were identified in peptides structures of PRLGs, however, FfSte3.s6 contained only two types, suggests its difference in evolution and function. We have further analyzed the expression patterns of each PRLGs in different developmental stages of the fruiting body in F. filiformis by quantitative real-time polymerase chain reaction (qRT-PCR). The results exhibited expression variation of PRLGs at different developmental stages of the F. filiformis. Especially, FfSte3.s1 and FfSte3.s2 exhibited maximum expression level in mycelia stage. Other PRLGs exhibited high expression level in fruiting body stages. This study suggests that PRLGs could be vital genes involving in fruiting body development in F. filiformis. However, further studies could be performed to reveal their specific functional pathways in the fruiting body development" |
| Keywords: | "Amino Acid Sequence Flammulina/*genetics/growth & development/metabolism Fruiting Bodies, Fungal/genetics/growth & development/metabolism *Gene Expression Profiling *Gene Expression Regulation, Fungal Genes, Fungal/*genetics Mycelium/genetics/growth & dev;" |
| Notes: | "MedlineMeng, Li Chou, Tiansheng Jiang, Siyuan Wang, Li Zhu, Mengjuan Mukhtar, Irum Xie, Baogui Wang, Wei eng No. 31902086/National Natural Science Foundation of China/ No. 31701976/National Natural Science Foundation of China/ No. 31801920/National Natural Science Foundation of China/ ZR2018BC041/Natural Science Foundation of Shandong Province/ 2018YFD1001001/National Key Research and Development Program of China/ Germany 2020/07/29 Arch Microbiol. 2020 Dec; 202(10):2671-2678. doi: 10.1007/s00203-020-01990-0. Epub 2020 Jul 27" |
