| Title: | Analysis of Tea Geometrid (Ectropis grisescens) Pheromone Gland Extracts Using GC-EAD and GCxGC/TOFMS |
| Author(s): | Ma T; Xiao Q; Yu YG; Wang C; Zhu CQ; Sun ZH; Chen XY; Wen XJ; |
| Address: | "Guangdong Key Laboratory for Innovative Development and Utilization of Forest Plant Germplasm, College of Forestry and Landscape Architecture, South China Agricultural University , Guangzhou 510642, China. Key Laboratory of Tea Plants Biology and Resources Utilization of Agriculture Ministry, Tea Research Institute, Chinese Academy of Agricultural Sciences , Hangzhou 310008, China" |
| ISSN/ISBN: | 1520-5118 (Electronic) 0021-8561 (Linking) |
| Abstract: | "The tea geometrid, Ectropis grisescens Warren, is one of the most severe defoliator insect pests in tea plantations, China. The use of insecticides, etc., is forbidden on organic tea plantations. No female-produced sex pheromones of E. grisescens had been previously identified. In the present study, female gland extracts were analyzed by gas chromatography coupled with electroantennographic detection (GC-EAD) and two-dimensional gas chromatography (GCxGC) using a time-of-flight mass spectrometric detector (TOFMS). Two components, (Z,Z,Z)-3,6,9-octadecatriene (Z3Z6Z9-18:Hy) and (Z,Z)-3,9-6,7-epoxyoctadecadiene (Z3Z9-6,7-epo-18:Hy), were identified from pheromone gland extracts, and their electrophysiological and behavioral activity evaluated. Under laboratory conditions, Z3Z9-6,7-epo-18:Hy elicited a stronger electrophysiological response than Z3Z6Z9-18:Hy. In the field, traps baited with Z3Z9-6,7-epo-18:Hy alone showed better results than traps baited with Z3Z6Z9-18:Hy, and the binary mixture of Z3Z9-6,7-epo-18:Hy and Z3Z6Z9-18:Hy in a ratio of 4:1 (approximate ratio of females emitting pheromone) caught more males than the single components or any other blends. This study showed that Z3Z6Z9-18:Hy and Z3Z9-6,7-epo-18:Hy are the sex pheromone components of E. grisescens and that they prove useful in developing alternative management tools for the pest" |
| Keywords: | "Animals Chromatography, Gas/*methods Mass Spectrometry/*methods Moths/*chemistry Sex Attractants/*analysis Ectropis grisescens field bioassay identification sex pheromone;" |
| Notes: | "MedlineMa, Tao Xiao, Qiang Yu, Yu-Geng Wang, Cai Zhu, Cheng-Qi Sun, Zhao-Hui Chen, Xiao-Yang Wen, Xiu-Jun eng 2016/04/05 J Agric Food Chem. 2016 Apr 27; 64(16):3161-6. doi: 10.1021/acs.jafc.6b00339. Epub 2016 Apr 11" |
