| Title: | Isolation and identification of aroma producing strain with esterification capacity from yellow water |
| Author(s): | Lai YT; Cheng KC; Lai CN; Lai YJ; |
| Address: | "Institute of Biotechnology, National Taiwan University, Taipei, Taiwan. Graduate Institute of Food Science Technology, National Taiwan University, Taipei, Taiwan. Department of Medical Research, China Medical University Hospital, China Medical University, Taichung, Taiwan. Department of Food Science, National Quemoy University, Kinmen, Taiwan" |
| DOI: | 10.1371/journal.pone.0211356 |
| ISSN/ISBN: | 1932-6203 (Electronic) 1932-6203 (Linking) |
| Abstract: | "Kaoliang is a refreshing fragranced type of Chinese spirits with slight apple fragrance that comes from ethyl acetate (EA). Special aromas are produced by esterification microorganisms, which affect the taste and quality of the wine. In this study, new yeast strains were isolated from yellow water, a by-product during fermentation process. Meanwhile, the optimal culture condition was determined for its growth and EA production. Three new strains, Kazachstaniaexigua, Candida humilis and Saccharomyces cerevisiae were identified from yellow water. Among these strains, S. cerevisiae S5 was the new and dominant strain. Results from response surface methodology showed that S. cerevisiae S5 produced 161.88 ppm of EA, in the medium with 4.91% yeast extract, 9.82% peptone, and 20.91% glucose after 96 hours of cultivation at 27.53 degrees C. GC analysis showed that aroma compounds, such as EA, isoamyl acetate and 2-phenylethanol increased from the sample of optimal condition when compared to the one from initial fermentation condition" |
| Keywords: | "Acetates/analysis/*metabolism Batch Cell Culture Techniques Candida/*chemistry/isolation & purification/metabolism Chromatography, Gas Rivers/*microbiology Saccharomyces cerevisiae/*chemistry/isolation & purification/metabolism Saccharomycetales/*chemistr;" |
| Notes: | "MedlineLai, Yen-Tso Cheng, Kuan-Chen Lai, Chia-Nuan Lai, Ying-Jang eng Research Support, Non-U.S. Gov't 2019/02/15 PLoS One. 2019 Feb 14; 14(2):e0211356. doi: 10.1371/journal.pone.0211356. eCollection 2019" |
