| Title: | "Molecular Characterization of MbraOR16, a Candidate Sex Pheromone Receptor in Mamestra brassicae (Lepidoptera: Noctuidae)" |
| Author(s): | Koblos G; Francois MC; Monsempes C; Montagne N; Fonagy A; Jacquin-Joly E; |
| Address: | "Plant Protection Institute, Centre for Agricultural Research, Hungarian Academy of Sciences, Budapest, Hungary. Inra, Sorbonne Universite, CNRS, IRD, UPEC, Universite Paris Diderot, Institute of Ecology and Environmental Sciences of Paris, Paris and Versailles, France" |
| ISSN/ISBN: | 1536-2442 (Electronic) 1536-2442 (Linking) |
| Abstract: | "Sex pheromone communication in Lepidoptera has long been a valuable model system for studying fundamental aspects of olfaction and its study has led to the establishment of environmental-friendly pest control strategies. The cabbage moth, Mamestra brassicae (Linnaeus) (Lepidoptera: Noctuidae), is a major pest of Cruciferous vegetables in Europe and Asia. Its sex pheromone has been characterized and is currently used as a lure to trap males; however, nothing is known about the molecular mechanisms of sex pheromone reception in male antennae. Using homology cloning and rapid amplification of cDNA ends-PCR strategies, we identified the first candidate pheromone receptor in this species. The transcript was specifically expressed in the antennae with a strong male bias. In situ hybridization experiments within the antennae revealed that the receptor-expressing cells were closely associated with the olfactory structures, especially the long trichoid sensilla known to be pheromone-sensitive. The deduced protein is predicted to adopt a seven-transmembrane structure, a hallmark of insect odorant receptors, and phylogenetically clustered in a clade that grouped a majority of the Lepidoptera pheromone receptors characterized to date. Taken together, our data support identification of a candidate pheromone receptor and provides a basis for better understanding how this species detects a signal critical for reproduction" |
| Keywords: | "Amino Acid Sequence Animals Arthropod Antennae/*metabolism Base Sequence Insect Proteins/*genetics/metabolism Male Moths/*genetics/metabolism Phylogeny Receptors, Pheromone/*genetics/metabolism Sequence Alignment Sex Attractants/*metabolism;" |
| Notes: | "MedlineKoblos, Gabriella Francois, Marie-Christine Monsempes, Christelle Montagne, Nicolas Fonagy, Adrien Jacquin-Joly, Emmanuelle eng 2018/09/25 J Insect Sci. 2018 Sep 1; 18(5):5. doi: 10.1093/jisesa/iey090" |
