« Previous Abstract"'Aqua-space', a new headspace method for isolation of natural floral aromas using humidified air as a carrier gas"    Next AbstractEssential compounds in herbivore-induced plant volatiles that attract the predatory mite Neoseiulus womersleyi »

Chem Senses

Title:		Modification of synapse formation of accessory olfactory bulb neurons by coculture with vomeronasal neurons
Author(s):		Ishimatsu Y; Moriya-Ito K; Muramoto K; Ichikawa M;
Address:		"Laboratory of Anatomy and Cell Biology, Department of Neuroscience Basic Technology, Tokyo Metropolitan Institute for Neuroscience, 2-6 Musashidai, Fuchu, Tokyo 183-8526, Japan"
Journal Title:		Chem Senses
Year:		2006
Volume:		20060301
Issue:		4
Page Number:		371 - 378
DOI:		10.1093/chemse/bjj041
ISSN/ISBN:		0379-864X (Print) 0379-864X (Linking)
Abstract:		"Previously, a coculture system of accessory olfactory bulb (AOB) neurons and vomeronasal (VN) neurons was established for studying the functional roles of AOB neurons in pheromonal signal processing. In this study, the effect of VN neurons on the development of AOB neurons was examined in a coculture system. Spine density was quantitatively measured for various culture periods of 21, 28, 36, and 42 days in vitro. The densities of dendritic spines were lower in the coculture than in single culture for all periods in vitro. Synapse formation on spines was analyzed immunocytochemically using an anti-synaptophysin antibody. The ratio of the density of synaptophysin-immunopositive spine/total spine density was larger in the coculture than in the single culture. The volume of spine head was larger in the coculture than in single culture. These changes were not observed in the coculture in which there was no physical contact between AOB neurons and VN neurons. These observations suggest that synapse formation on the spines of AOB neurons is modified by physical contact with VN neurons"
Keywords:		"Actins/chemistry/metabolism Animals Cells, Cultured Coculture Techniques Dendritic Spines/physiology/ultrastructure Green Fluorescent Proteins/chemistry/metabolism Olfactory Bulb/*cytology/physiology Olfactory Receptor Neurons/*cytology/metabolism/physiol;neuroscience;"
Notes:		"MedlineIshimatsu, Yuuki Moriya-Ito, Keiko Muramoto, Kazuyo Ichikawa, Masumi eng Research Support, Non-U.S. Gov't England 2006/03/03 Chem Senses. 2006 May; 31(4):371-8. doi: 10.1093/chemse/bjj041. Epub 2006 Mar 1"