| Title: | Ca2+ imaging of identifiable neurons labeled by electroporation in insect brains |
| Author(s): | Fujiwara T; Kazawa T; Haupt SS; Kanzaki R; |
| Address: | "Graduate School of Information Science and Technology, The University of Tokyo, Tokyo, Japan" |
| DOI: | 10.1097/WNR.0b013e32832e7d93 |
| ISSN/ISBN: | 1473-558X (Electronic) 0959-4965 (Linking) |
| Abstract: | "Identifiable neurons in the silkmoth brain were studied physiologically after loading of Ca indicator by local electroporation. Small groups of neurons with projections in confined regions of the antennal lobe were labeled, and Ca imaging showed differences in the dose-response characteristics between projection neurons of the macroglomerular complex that responded to different pheromone components. Compared with projection neurons, local interneurons showed shorter response latencies. Targeted labeling of neurons innervating restricted regions of neuropil by local electroporation is a powerful method that will be generally useful for elucidating details of the functional circuitry in insect brains" |
| Keywords: | "Animals Bombyx/cytology/*physiology Brain/cytology Calcium/*metabolism Electroporation Fluorescent Dyes Interneurons/physiology Male Microscopy, Confocal Microscopy, Fluorescence Neurons/*physiology Odorants Olfactory Perception Physical Stimulation Time;" |
| Notes: | "MedlineFujiwara, Terufumi Kazawa, Tomoki Haupt, Stephan Shuichi Kanzaki, Ryohei eng Research Support, Non-U.S. Gov't England 2009/06/25 Neuroreport. 2009 Aug 5; 20(12):1061-5. doi: 10.1097/WNR.0b013e32832e7d93" |
