| Title: | Inter-kingdom relationships in Crohn's disease explored using a multi-omics approach |
| Author(s): | Frau A; Ijaz UZ; Slater R; Jonkers D; Penders J; Campbell BJ; Kenny JG; Hall N; Lenzi L; Burkitt MD; Pierik M; Darby AC; Probert CSJ; |
| Address: | "Department of Molecular and Clinical Cancer Medicine, Institute of Systems, Molecular and Integrative Biology, University of Liverpool, Liverpool, UK. School of Engineering, University of Glasgow, Glasgow, UK. School of Nutrition and Translational Research in Metabolism, Maastricht University, Maastricht, Netherlands. Department of Infection & Microbiomes, University of Liverpool, Liverpool, UK. Teagasc Food Research Centre, Cork, Ireland. Earlham Institute, Norwich, UK. School of Biological Sciences, University of East Anglia, Norwich, Norfolk, UK. Centre for Genomic Research, University of Liverpool, Liverpool, UK. Division of Diabetes, Endocrinology and Gastroenterology, University of Manchester, Manchester, UK" |
| DOI: | 10.1080/19490976.2021.1930871 |
| ISSN/ISBN: | 1949-0984 (Electronic) 1949-0976 (Print) 1949-0976 (Linking) |
| Abstract: | "The etiology of Crohn's disease (CD) is multifactorial. Bacterial and fungal microbiota are involved in the onset and/or progression of the disease. A bacterial dysbiosis in CD patients is accepted; however, less is known about the mycobiome and the relationships between the two communities. We investigated the interkingdom relationships, their metabolic consequences, and the changes in the fungal community during relapse and remission in CD.Two cohorts were evaluated: a British cohort (n = 63) comprising CD and ulcerative colitis patients, and controls. The fungal and bacterial communities of biopsy and fecal samples were analyzed, with the fecal volatiles; datasets were also integrated; and a Dutch cohort (n = 41) comprising CD patients and healthy controls was analyzed for stability of the gut mycobiome.A dysbiosis of the bacterial community was observed in biopsies and stool. Results suggest Bacteroides is likely key in CD and may modulate Candida colonization. A dysbiosis of the fungal community was observed only in the Dutch cohort; Malassezia and Candida were increased in patients taking immunosuppressants. Longitudinal analysis showed an increase in Cyberlindnera in relapse. Saccharomyces was dominant in all fecal samples, but not in biopsies, some of which did not yield fungal reads; amino acid degradation was the main metabolic change associated with CD and both bacteria and fungi might be implicated.We have shown that Bacteroides and yeasts may play a role in CD; understanding their role and relationship in the disease would shed new light on the development and treatment of CD" |
| Keywords: | Adolescent Adult Aged Bacteria/classification/genetics/*isolation & purification Child Cohort Studies Crohn Disease/*microbiology Dysbiosis/microbiology Feces/microbiology Female Fungi/classification/genetics/*isolation & purification *Gastrointestinal Mi; |
| Notes: | "MedlineFrau, Alessandra Ijaz, Umer Z Slater, Rachael Jonkers, Daisy Penders, John Campbell, Barry J Kenny, John G Hall, Neil Lenzi, Luca Burkitt, Michael D Pierik, Marieke Darby, Alistair C Probert, Christopher S J eng Observational Study Research Support, Non-U.S. Gov't 2021/07/10 Gut Microbes. 2021 Jan-Dec; 13(1):1930871. doi: 10.1080/19490976.2021.1930871" |
