| Title: | "Sex pheromone receptor specificity in the European corn borer moth, Ostrinia nubilalis" |
| Author(s): | Wanner KW; Nichols AS; Allen JE; Bunger PL; Garczynski SF; Linn CE; Robertson HM; Luetje CW; |
| Address: | "Department of Plant Sciences and Plant Pathology, Montana State University, Bozeman, Montana, USA. kwanner@montana.edu" |
| DOI: | 10.1371/journal.pone.0008685 |
| ISSN/ISBN: | 1932-6203 (Electronic) 1932-6203 (Linking) |
| Abstract: | "BACKGROUND: The European corn borer (ECB), Ostrinia nubilalis (Hubner), exists as two separate sex pheromone races. ECB(Z) females produce a 97ratio3 blend of Z11- and E11-tetradecenyl acetate whereas ECB(E) females produce an opposite 1ratio99 ratio of the Z and E isomers. Males of each race respond specifically to their conspecific female's blend. A closely related species, the Asian corn borer (ACB), O. furnacalis, uses a 3ratio2 blend of Z12- and E12-tetradecenyl acetate, and is believed to have evolved from an ECB-like ancestor. To further knowledge of the molecular mechanisms of pheromone detection and its evolution among closely related species we identified and characterized sex pheromone receptors from ECB(Z). METHODOLOGY: Homology-dependent (degenerate PCR primers designed to conserved amino acid motifs) and homology-independent (pyrophosphate sequencing of antennal cDNA) approaches were used to identify candidate sex pheromone transcripts. Expression in male and female antennae was assayed by quantitative real-time PCR. Two-electrode voltage clamp electrophysiology was used to functionally characterize candidate receptors expressed in Xenopus oocytes. CONCLUSION: We characterized five sex pheromone receptors, OnOrs1 and 3-6. Their transcripts were 14-100 times more abundant in male compared to female antennae. OnOr6 was highly selective for Z11-tetradecenyl acetate (EC(50) = 0.86+/-0.27 microM) and was at least three orders of magnitude less responsive to E11-tetradecenyl acetate. Surprisingly, OnOr1, 3 and 5 responded to all four pheromones tested (Z11- and E11-tetradecenyl acetate, and Z12- and E12-tetradecenyl acetate) and to Z9-tetradecenyl acetate, a behavioral antagonist. OnOr1 was selective for E12-tetradecenyl acetate based on an efficacy that was at least 5-fold greater compared to the other four components. This combination of specifically- and broadly-responsive pheromone receptors corresponds to published results of sensory neuron activity in vivo. Receptors broadly-responsive to a class of pheromone components may provide a mechanism for variation in the male moth response that enables population level shifts in pheromone blend use" |
| Keywords: | "Animals Female Male *Moths/physiology Polymerase Chain Reaction Receptors, Odorant/*metabolism Sex Attractants/*metabolism Sexual Behavior, Animal;" |
| Notes: | "MedlineWanner, Kevin W Nichols, Andrew S Allen, Jean E Bunger, Peggy L Garczynski, Stephen F Linn, Charles E Robertson, Hugh M Luetje, Charles W eng R01 DC008119/DC/NIDCD NIH HHS/ R01 DC011091/DC/NIDCD NIH HHS/ DC008119/DC/NIDCD NIH HHS/ Research Support, N.I.H., Extramural Research Support, U.S. Gov't, Non-P.H.S. 2010/01/20 PLoS One. 2010 Jan 13; 5(1):e8685. doi: 10.1371/journal.pone.0008685" |
