| Title: | Sex Pheromone Receptor-Derived Peptide Biosensor for Efficient Monitoring of the Cotton Bollworm Helicoverpa armigera |
| Author(s): | Wang Z; Ma W; Gao Z; Zhao Z; Du B; Wei J; Jiang D; Lan K; Chen R; Yan S; Qin G; |
| Address: | "School of Microelectronics, Tianjin Key Laboratory of Imaging and Sensing Microelectronic Technology, Tianjin University, Tianjin 300072, P. R. China. College of Forestry, Northeast Forestry University, Harbin 150040, Heilongjiang, P. R. China. Key Laboratory of Structure-Based Drug Design & Discovery, Ministry of Education, Shenyang Pharmaceutical University, Shenyang 110016, Liaoning, P. R. China. School of Pharmaceutical Science and Technology, Tianjin University, Tianjin 300072, P. R. China. Shanghe County Agriculture and Rural Enterprise Development Center, Jinan 251600, Shandong, P. R. China" |
| DOI: | 10.1021/acssensors.2c02384 |
| ISSN/ISBN: | 2379-3694 (Electronic) 2379-3694 (Linking) |
| Abstract: | "The cotton bollworm, Helicoverpa armigera (H. armigera), causes damage to a wide range of cultivated crops and is one of the pests with the greatest economic importance for global agriculture. Currently, the detection of H. armigera is based on manual sampling. A low limit of detection (LOD), convenient, and real-time monitoring method is urgently needed for its early warning and efficient management. Here, we characterized the amino acid sequence in the sex pheromone receptors (SPRs) recognizing the pheromone components of H. armigera by three-dimensional (3D) modeling and molecular docking. Next, sex pheromone receptor-derived peptides (SPRPs) were synthesized and conjugated to nanotubes by chemical connection. The modified nanotubes were used to fabricate a sensor capable of real-time monitoring of gaseous sex pheromone compounds with a low LOD ( approximately 10 ppb for Z11-16:Ald) and selectivity, and the sensor was able to detect a single live H. armigera. Furthermore, the developed biosensor allowed direct monitoring of the pheromone release dynamics by female H. armigera and showed that the release was instantly reduced in response to light. Here, we report the first demonstration of a biosensing method for detecting gaseous sex pheromones and live H. armigera. The findings show the great potential of the SPRP sensor for broad applications in insect biology study and infestation monitoring" |
| Keywords: | "Animals Female *Sex Attractants/metabolism Receptors, Pheromone/metabolism Molecular Docking Simulation *Moths/metabolism Peptides Helicoverpa armigera gas sensor monitor sex pheromone sex pheromone receptor-derived peptide;" |
| Notes: | "MedlineWang, Zhi Ma, Weichao Gao, Zisen Zhao, Zhihan Du, Baojiang Wei, Junqing Jiang, Dun Lan, Kuibo Chen, Ruibing Yan, Shanchun Qin, Guoxuan eng Research Support, Non-U.S. Gov't 2023/01/07 ACS Sens. 2023 Jan 27; 8(1):363-371. doi: 10.1021/acssensors.2c02384. Epub 2023 Jan 6" |
