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J Breath Res

Title:		Regeneration of insulin-producing pancreatic cells using a volatile bioactive compound and human teeth
Author(s):		Okada M; Imai T; Yaegaki K; Ishkitiev N; Tanaka T;
Address:		"Nippon Dental University, School of Life Dentistry at Tokyo, Department of Oral Health, 1-9-20 Chiyoda-ku, 102-8159 Tokyo, Japan"
Journal Title:		J Breath Res
Year:		2014
Volume:		20141030
Issue:		4
Page Number:		46004 -
DOI:		10.1088/1752-7155/8/4/046004
ISSN/ISBN:		1752-7163 (Electronic) 1752-7155 (Linking)
Abstract:		"Transplantation of insulin (INS)-secreting cells differentiated in vitro from stem cells promises a safer and easier treatment of severe diabetes mellitus. A volatile bioactive compound, hydrogen sulfide (H2S), promotes cell differentiation; human tooth-pulp stem cells undergo hepatic differentiation. The aim of this study is to develop a novel protocol using H2S to enhance pancreatic differentiation from the CD117(+) cell fraction of human tooth pulp. During the differentiation, the cells were exposed to 0.1 ng ml(-1) H2S. Immunocytochemistry, RT-PCR, determination of INS c-peptide content and flow cytometry of pancreatically related markers were carried out. Expression of WNT and the PI3K/AKT signaling pathway were also determined by PCR array. After differentiation, INS, glucagon (GCG), somatostatin (SST) and pancreatic polypeptide (PPY) were positive when examined by immunofluorescence. INS and GCG were also determined flow-cytometrically. Only the cells expressing INS increased after H2S exposure. The number of cells expressing GCG was significantly decreased. Genes involved in canonical WNT and the WNT/calcium pathways were highly expressed after H2S exposure. H2S accelerated INS synthesis and secretion by regenerated INS-producing cells from human teeth. All signaling pathway functions of the PI3K-AKT pathway were extremely activated by H2S exposure. The matured INS-producing cells originating in human teeth were increased by H2S in order to control blood-glucose level"
Keywords:		C-Peptide/metabolism Cell Differentiation/drug effects Flow Cytometry Gene Expression Regulation/drug effects Glucose/pharmacology Humans Hydrogen Sulfide/pharmacology Immunohistochemistry Insulin/*biosynthesis/metabolism Insulin Secretion Insulin-Secreti;
Notes:		"MedlineOkada, Mio Imai, Toshio Yaegaki, Ken Ishkitiev, Nikolay Tanaka, Tomoko eng Research Support, Non-U.S. Gov't England 2014/11/02 J Breath Res. 2014 Oct 30; 8(4):046004. doi: 10.1088/1752-7155/8/4/046004"