| Title: | Ste18p is a positive control element in the mating process of Candida albicans |
| Author(s): | Lu H; Sun Y; Jiang YY; Whiteway M; |
| Address: | "Center for New Drug Research, Department of Pharmacology, School of Pharmacy, Second Military Medical University, Shanghai, China" |
| ISSN/ISBN: | 1535-9786 (Electronic) 1535-9778 (Print) 1535-9786 (Linking) |
| Abstract: | "Heterotrimeric G proteins are an important class of eukaryotic signaling molecules that have been identified as central elements in the pheromone response pathways of many fungi. In the fungal pathogen Candida albicans, the STE18 gene (ORF19.6551.1) encodes a potential gamma subunit of a heterotrimeric G protein; this protein contains the C-terminal CAAX box characteristic of gamma subunits and has sequence similarity to gamma subunits implicated in the mating pathways of a variety of fungi. Disruption of this gene was shown to cause sterility of MTLa mating cells and to block pheromone-induced gene expression and shmoo formation; deletion of just the CAAX box residues is sufficient to inactivate Ste18 function in the mating process. Intriguingly, ectopic expression behind the strong ACT1 promoter of either the Galpha or the Gbeta subunit of the heterotrimeric G protein is able to suppress the mating defect caused by deletion of the Ggamma subunit and restore both pheromone-induced gene expression and morphology changes" |
| Keywords: | "Amino Acid Sequence Candida albicans/*genetics/metabolism Fungal Proteins/*genetics/metabolism GTP-Binding Protein gamma Subunits/*genetics/metabolism Gene Deletion *Gene Expression Regulation, Fungal Genes, Mating Type, Fungal Molecular Sequence Data Phe;" |
| Notes: | "MedlineLu, Hui Sun, Yuan Jiang, Yuan-Ying Whiteway, Malcolm eng 42516-4/Canadian Institutes of Health Research/Canada MOP 42516/CAPMC/CIHR/Canada Research Support, Non-U.S. Gov't 2014/02/04 Eukaryot Cell. 2014 Apr; 13(4):461-9. doi: 10.1128/EC.00320-13. Epub 2014 Jan 31" |
