| Title: | Wolbachia infections of the butterfly Eurema mandarina interfere with embryonic development of the sawfly Athalia rosae |
| Author(s): | Kageyama D; Wang CH; Hatakeyama M; |
| Address: | "Institute of Agrobiological Sciences, National Agriculture and Food Research Organization (NARO), Tsukuba, Ibaraki 305-8634, Japan. Electronic address: kagymad@affrc.go.jp. Department of Entomology, College of Bioresources and Agriculture, National Taiwan University, Taipei 106, Taiwan, ROC. Electronic address: wangch@ntu.edu.tw. Institute of Agrobiological Sciences, National Agriculture and Food Research Organization (NARO), Tsukuba, Ibaraki 305-8634, Japan. Electronic address: sawfly@affrc.go.jp" |
| DOI: | 10.1016/j.jip.2017.08.003 |
| ISSN/ISBN: | 1096-0805 (Electronic) 0022-2011 (Linking) |
| Abstract: | "Although maternally transmitted microorganisms such as Wolbachia are well known to have a variety of effects on the reproduction of diverse insect species, little is known about the underlying mechanisms of actions. Artificial transfer of Wolbachia between taxonomically distant host species may provide insights into Wolbachia-induced manipulations of hosts. Here we performed a cross-order transfer of feminizing Wolbachia derived from a butterfly, Eurema mandarina. The Wolbachia were propagated in the Eurema hecabe cell line, called NTU-YB, and then used to inject prepupal/pupal females of a Wolbachia-free hymenopteran sawfly, Athalia rosae. The 14 females that emerged as adults looked morphologically and behaviorally healthy, and ovarian development appeared normal on dissection. However, in contrast to the control, none of the 333 eggs harbored by the seven Wolbachia-injected females developed successfully. Similarly, none of the 140 eggs laid on host plant by the four Wolbachia-injected females, which were mated with males, showed any signs of development. Wolbachia infection was detected from whole-body samples of the inoculated individuals, but not from the eggs they produced. Disruption of embryonic development despite the absence of Wolbachia in the egg cytoplasm may represent a new phenotype involving maternal effects that result in female sterility" |
| Keywords: | Animals Bacterial Infections/*veterinary Butterflies/*microbiology Embryonic Development/*physiology Female Hymenoptera/*microbiology Male Wolbachia Athalia rosae Embryogenesis Eurema hecabe Eurema mandarina Transinfection; |
| Notes: | "MedlineKageyama, Daisuke Wang, Chung-Hsiung Hatakeyama, Masatsugu eng Research Support, Non-U.S. Gov't 2017/08/10 J Invertebr Pathol. 2017 Nov; 150:76-81. doi: 10.1016/j.jip.2017.08.003. Epub 2017 Aug 5" |
