| Title: | The SIR2/3/4 complex and SIR2 alone promote longevity in Saccharomyces cerevisiae by two different mechanisms |
| Author(s): | Kaeberlein M; McVey M; Guarente L; |
| Address: | "Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA" |
| ISSN/ISBN: | 0890-9369 (Print) 0890-9369 (Linking) |
| Abstract: | "The SIR genes are determinants of life span in yeast mother cells. Here we show that life span regulation by the Sir proteins is independent of their role in nonhomologous end joining. The short life span of a sir3 or sir4 mutant is due to the simultaneous expression of a and alpha mating-type information, which indirectly causes an increase in rDNA recombination and likely increases the production of extrachromosomal rDNA circles. The short life span of a sir2 mutant also reveals a direct failure to repress recombination generated by the Fob1p-mediated replication block in the rDNA. Sir2p is a limiting component in promoting yeast longevity, and increasing the gene dosage extends the life span in wild-type cells. A possible role of the conserved SIR2 in mammalian aging is discussed" |
| Keywords: | "DNA, Fungal DNA, Ribosomal DNA-Binding Proteins/genetics/*physiology Fungal Proteins/genetics/*physiology *Histone Deacetylases Mating Factor Mutagenesis Peptides Phenotype Saccharomyces cerevisiae/genetics/*physiology *Saccharomyces cerevisiae Proteins *;" |
| Notes: | "MedlineKaeberlein, M McVey, M Guarente, L eng T32 GM007287/GM/NIGMS NIH HHS/ T32 GM07287/GM/NIGMS NIH HHS/ Research Support, U.S. Gov't, P.H.S. 1999/10/16 Genes Dev. 1999 Oct 1; 13(19):2570-80. doi: 10.1101/gad.13.19.2570" |
