| Title: | The yeast Ste2p G protein-coupled receptor dimerizes on the cell plasma membrane |
| Author(s): | Cevheroglu O; Kumas G; Hauser M; Becker JM; Son CD; |
| Address: | "Department of Biological Sciences, Middle East Technical University, Universiteler Mah. Dumlupinar Blv. No: 1, 06800 Cankaya, Ankara, Turkey; Department of Microbiology, University of Tennessee, Knoxville, TN 37996, United States. Department of Biological Sciences, Middle East Technical University, Universiteler Mah. Dumlupinar Blv. No: 1, 06800 Cankaya, Ankara, Turkey. Department of Microbiology, University of Tennessee, Knoxville, TN 37996, United States. Department of Biological Sciences, Middle East Technical University, Universiteler Mah. Dumlupinar Blv. No: 1, 06800 Cankaya, Ankara, Turkey. Electronic address: cson@metu.edu.tr" |
| Journal Title: | Biochim Biophys Acta Biomembr |
| DOI: | 10.1016/j.bbamem.2017.01.008 |
| ISSN/ISBN: | 0005-2736 (Print) 0005-2736 (Linking) |
| Abstract: | "Dimerization of G protein-coupled receptors (GPCR) may play an important role in maturation, internalization, signaling and/or pharmacology of these receptors. However, the location where dimerization occurs is still under debate. In our study, variants of Ste2p, a yeast mating pheromone GPCR, were tagged with split EGFP (enhanced green fluorescent protein) fragments inserted between transmembrane domain seven and the C-terminus or appended to the C-terminus. Bimolecular Fluorescence Complementation (BiFC) assay was used to determine where receptor dimerization occurred during protein trafficking by monitoring generation of EGFP fluorescence, which occurred upon GPCR dimerization. Our results suggest that these tagged receptors traffic to the membrane as monomers, undergo dimerization or higher ordered oligomerization predominantly on the plasma membrane, and are internalized as dimers/oligomers. This study is the first to provide direct in vivo visualization of GPCR dimerization/oligomerization, during trafficking to and from the plasma membrane" |
| Keywords: | "Cell Membrane/*chemistry *Protein Multimerization Receptors, G-Protein-Coupled/*chemistry Receptors, Mating Factor/*chemistry Saccharomyces cerevisiae Proteins/*chemistry Bimolecular fluorescence complementation assay (BiFC) GPCR dimerization GPCR traffic;" |
| Notes: | "MedlineCevheroglu, Orkun Kumas, Gozde Hauser, Melinda Becker, Jeffrey M Son, Cagdas D eng GM112496/NH/NIH HHS/ Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Netherlands 2017/01/12 Biochim Biophys Acta Biomembr. 2017 May; 1859(5):698-711. doi: 10.1016/j.bbamem.2017.01.008. Epub 2017 Jan 8" |
