| Title: | The protein pheromone Er-1 of the ciliate Euplotes raikovi stimulates human T-cell activity: involvement of interleukin-2 system |
| Author(s): | Cervia D; Catalani E; Belardinelli MC; Perrotta C; Picchietti S; Alimenti C; Casini G; Fausto AM; Vallesi A; |
| Address: | "Department for Innovation in Biological, Agro-food and Forest systems (DIBAF), University of Tuscia, Viterbo, Italy. d.cervia@unitus.it" |
| DOI: | 10.1016/j.yexcr.2012.10.007 |
| ISSN/ISBN: | 1090-2422 (Electronic) 0014-4827 (Linking) |
| Abstract: | "Water-soluble protein signals (pheromones) of the ciliate Euplotes have been supposed to be functional precursors of growth factors and cytokines that regulate cell-cell interaction in multi-cellular eukaryotes. This work provides evidence that native preparations of the Euplotes raikovi pheromone Er-1 (a helical protein of 40 amino acids) specifically increases viability, DNA synthesis, proliferation, and the production of interferon-gamma, tumor necrosis factor-alpha, interleukin (IL)-1beta, IL-2, and IL-13 in human Jurkat T-cells. Also, Er-1 significantly decreases the mRNA levels of the beta and gamma subunits of IL-2 receptor (IL-2R), while the mRNA levels of the alpha subunit appeared to be not affected. Jurkat T-cell treatments with Er-1 induced the down-regulation of the IL-2Ralpha subunit by a reversible and time-dependent endocytosis, and increased the levels of phosphorylation of the extracellular signal-regulated kinases (ERK). The cell-type specificity of these effects was supported by the finding that Er-1, although unable to directly influence the growth of human glioma U-373 cells, induced Jurkat cells to synthesize and release factors that, in turn, inhibited the U-373 cell proliferation. Overall, these findings imply that Er-1 coupling to IL-2R and ERK immuno-enhances T-cell activity, and that this effect likely translates to an inhibition of glioma cell growth" |
| Keywords: | Animals Cell Proliferation/drug effects Ciliophora/chemistry/immunology/metabolism Euplotes/chemistry/immunology/metabolism Gene Expression Regulation/drug effects Glioma/immunology/pathology Humans Interleukin-2/*physiology Jurkat Cells Lymphocyte Activa; |
| Notes: | "MedlineCervia, Davide Catalani, Elisabetta Belardinelli, Maria Cristina Perrotta, Cristiana Picchietti, Simona Alimenti, Claudio Casini, Giovanni Fausto, Anna Maria Vallesi, Adriana eng Evaluation Study Research Support, Non-U.S. Gov't 2012/10/30 Exp Cell Res. 2013 Feb 1; 319(3):56-67. doi: 10.1016/j.yexcr.2012.10.007. Epub 2012 Oct 24" |
