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J Forensic Sci

Title:		Volatile Organic Compound Profiling from Postmortem Microbes using Gas Chromatography-Mass Spectrometry
Author(s):		Cernosek T; Eckert KE; Carter DO; Perrault KA;
Address:		"Laboratory of Forensic and Bioanalytical Chemistry, Forensic Sciences Unit, Division of Natural Sciences and Mathematics, Chaminade University of Honolulu, 3140 Waialae Avenue, Honolulu, HI. Laboratory of Forensic Taphonomy, Forensic Sciences Unit, Division of Natural Sciences and Mathematics, Chaminade University of Honolulu, 3140 Waialae Avenue, Honolulu, HI"
Journal Title:		J Forensic Sci
Year:		2020
Volume:		20190903
Issue:		1
Page Number:		134 - 143
DOI:		10.1111/1556-4029.14173
ISSN/ISBN:		1556-4029 (Electronic) 0022-1198 (Linking)
Abstract:		"Volatile organic compounds (VOCs) are by-products of cadaveric decomposition and are responsible for the odor associated with decomposing remains. The direct link between VOC production and individual postmortem microbes has not been well characterized experimentally. The purpose of this study was to profile VOCs released from three postmortem bacterial isolates (Bacillus subtilis, Ignatzschineria indica, I. ureiclastica) using solid-phase microextraction arrow (SPME Arrow) and gas chromatography-mass spectrometry (GC-MS). Species were inoculated in headspace vials on Standard Nutrient Agar and monitored over 5 days at 24 degrees C. Each species exhibited a different VOC profile that included common decomposition VOCs. VOCs exhibited upward or downward temporal trends over time. Ignatzschineria indica produced a large amount of dimethyldisulfide. Other compounds of interest included alcohols, aldehydes, aromatics, and ketones. This provides foundational data to link decomposition odor with specific postmortem microbes to improve understanding of underlying mechanisms for decomposition VOC production"
Keywords:		Animals Bacillus subtilis/*metabolism Forensic Medicine Gammaproteobacteria/*metabolism Gas Chromatography-Mass Spectrometry *Postmortem Changes Solid Phase Microextraction Swine Volatile Organic Compounds/*metabolism cadaver decomposition decomposition o;
Notes:		"MedlineCernosek, Terezie Eckert, Kevin E Carter, David O Perrault, Katelynn A eng 1752607/National Science Foundation/ 2019/09/04 J Forensic Sci. 2020 Jan; 65(1):134-143. doi: 10.1111/1556-4029.14173. Epub 2019 Sep 3"