| Title: | Virus-induced breath biomarkers: A new perspective to study the metabolic responses of COVID-19 vaccinees |
| Author(s): | Cen Z; Lu B; Ji Y; Chen J; Liu Y; Jiang J; Li X; Li X; |
| Address: | "Department of Environmental Science & Engineering, Fudan University, Shanghai, 200438, PR China. Institute of Mass Spectrometry and Atmospheric Environment, Jinan University, Guangzhou, 510632, PR China. Department of Environmental Science & Engineering, Fudan University, Shanghai, 200438, PR China. Electronic address: lixiang@fudan.edu.cn" |
| DOI: | 10.1016/j.talanta.2023.124577 |
| ISSN/ISBN: | 1873-3573 (Electronic) 0039-9140 (Print) 0039-9140 (Linking) |
| Abstract: | "Coronavirus disease 2019 (COVID-19) vaccines can protect people from the infection; however, the action mechanism of vaccine-mediated metabolism remains unclear. Herein, we performed breath tests in COVID-19 vaccinees that revealed metabolic reprogramming induced by protective immune responses. In total, 204 breath samples were obtained from COVID-19 vaccinees and non-vaccinated controls, wherein numerous volatile organic compounds (VOCs) were detected by comprehensive two-dimensional gas chromatography and time-of-flight mass spectrometry system. Subsequently, 12 VOCs were selected as biomarkers to construct a signature panel using alveolar gradients and machine learning-based procedure. The signature panel could distinguish vaccinees from control group with a high prediction performance (AUC, 0.9953; accuracy, 94.42%). The metabolic pathways of these biomarkers indicated that the host-pathogen interactions enhanced enzymatic activity and microbial metabolism in the liver, lung, and gut, potentially constituting the dominant action mechanism of vaccine-driven metabolic regulation. Thus, our findings of this study highlight the potential of measuring exhaled VOCs as rapid, non-invasive biomarkers of viral infections. Furthermore, breathomics appears as an alternative for safety evaluation of biological agents and disease diagnosis" |
| Keywords: | Humans *COVID-19/diagnosis Biomarkers/analysis Mass Spectrometry Machine Learning Breath Tests/methods *Volatile Organic Compounds/analysis Exhalation Biomarker Covid-19 Metabolism Vaccine Volatile organic compound; |
| Notes: | "MedlineCen, Zhengnan Lu, Bingqing Ji, Yongyan Chen, Jian Liu, Yongqian Jiang, Jiakui Li, Xue Li, Xiang eng Netherlands 2023/04/29 Talanta. 2023 Aug 1; 260:124577. doi: 10.1016/j.talanta.2023.124577. Epub 2023 Apr 22" |
