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Proc Natl Acad Sci U S A

Title:		Autoinducer binding by the quorum-sensing regulator TraR increases affinity for target promoters in vitro and decreases TraR turnover rates in whole cells
Author(s):		Zhu J; Winans SC;
Address:		"Section of Microbiology, Cornell University, Ithaca, NY 14853, USA"
Journal Title:		Proc Natl Acad Sci U S A
Year:		1999
Volume:		96
Issue:		9
Page Number:		4832 - 4837
DOI:		10.1073/pnas.96.9.4832
ISSN/ISBN:		0027-8424 (Print) 1091-6490 (Electronic) 0027-8424 (Linking)
Abstract:		TraR is an Agrobacterium transcriptional regulator whose activity requires the pheromone N-3-oxooctanoyl-L-homoserine lactone. TraR was purified as a complex with the pheromone and contained one pheromone molecule per protein monomer. TraR-pheromone complexes bound to a single DNA site and activated two promoters that flank this site. Promoter expression was elevated 30-fold by using a supercoiled template. Pheromone binding increased the affinity of TraR for this binding site. Pheromone also increased TraR abundance in vivo by causing a 20-fold decrease in TraR turnover rates
Keywords:		"Bacterial Proteins/*genetics DNA, Bacterial/genetics *Gene Expression Regulation, Bacterial Homoserine/*analogs & derivatives/genetics/metabolism Lactones/metabolism Pheromones/metabolism Promoter Regions, Genetic Rhizobium/*genetics/metabolism Transcript;"
Notes:		"MedlineZhu, J Winans, S C eng R01 GM042893/GM/NIGMS NIH HHS/ GM42893/GM/NIGMS NIH HHS/ Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. 1999/04/29 Proc Natl Acad Sci U S A. 1999 Apr 27; 96(9):4832-7. doi: 10.1073/pnas.96.9.4832"