« Previous Abstract"Consciousness, unconsciousness and death in the context of slaughter. Part I. Neurobiological mechanisms underlying stunning and killing"    Next AbstractAssessment of vancomycin resistance transfer among enterococci of clinical importance in milk matrix »

J Chem Ecol

Title:		Cell-Based Odorant Sensor Array for Odor Discrimination Based on Insect Odorant Receptors
Author(s):		Termtanasombat M; Mitsuno H; Misawa N; Yamahira S; Sakurai T; Yamaguchi S; Nagamune T; Kanzaki R;
Address:		"Graduate School of Information Science and Technology, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-8656, Japan. Research Center for Advanced Science and Technology, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo, 153-8904, Japan. Electronics-Inspired Interdisciplinary Research Institute, Toyohashi University of Technology, 1-1 Hibarigaoka, Tempaku-cho, Toyohashi, Aichi, 441-8580, Japan. Artificial Cell Membrane Systems Group, Kanagawa Academy of Science and Technology, KSP East 303, 3-2-1 Sakado, Takatsu-ku, Kawasaki, Kanagawa, 213-0012, Japan. Department of Chemistry and Biotechnology, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-8656, Japan. Graduate School of Information Science and Technology, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-8656, Japan. kanzaki@rcast.u-tokyo.ac.jp. Research Center for Advanced Science and Technology, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo, 153-8904, Japan. kanzaki@rcast.u-tokyo.ac.jp"
Journal Title:		J Chem Ecol
Year:		2016
Volume:		20160716
Issue:		7
Page Number:		716 - 724
DOI:		10.1007/s10886-016-0726-7
ISSN/ISBN:		1573-1561 (Electronic) 0098-0331 (Linking)
Abstract:		"The olfactory system of living organisms can accurately discriminate numerous odors by recognizing the pattern of activation of several odorant receptors (ORs). Thus, development of an odorant sensor array based on multiple ORs presents the possibility of mimicking biological odor discrimination mechanisms. Recently, we developed novel odorant sensor elements with high sensitivity and selectivity based on insect OR-expressing Sf21 cells that respond to target odorants by displaying increased fluorescence intensity. Here we introduce the development of an odorant sensor array composed of several Sf21 cell lines expressing different ORs. In this study, an array pattern of four cell lines expressing Or13a, Or56a, BmOR1, and BmOR3 was successfully created using a patterned polydimethylsiloxane film template and cell-immobilizing reagents, termed biocompatible anchor for membrane (BAM). We demonstrated that BAM could create a clear pattern of Sf21 sensor cells without impacting their odorant-sensing performance. Our sensor array showed odorant-specific response patterns toward both odorant mixtures and single odorant stimuli, allowing us to visualize the presence of 1-octen-3-ol, geosmin, bombykol, and bombykal as an increased fluorescence intensity in the region of Or13a, Or56a, BmOR1, and BmOR3 cell lines, respectively. Therefore, we successfully developed a new methodology for creating a cell-based odorant sensor array that enables us to discriminate multiple target odorants. Our method might be expanded into the development of an odorant sensor capable of detecting a large range of environmental odorants that might become a promising tool used in various applications including the study of insect semiochemicals and food contamination"
Keywords:		"Animals Cell Adhesion/drug effects Dimethylpolysiloxanes/pharmacology Humans Jurkat Cells Odorants/*analysis Receptors, Odorant/genetics/*metabolism Sf9 Cells Spodoptera Tissue Array Analysis/*methods Cell array patterning Insect odorant receptor Odor dis;"
Notes:		"MedlineTermtanasombat, Maneerat Mitsuno, Hidefumi Misawa, Nobuo Yamahira, Shinya Sakurai, Takeshi Yamaguchi, Satoshi Nagamune, Teruyuki Kanzaki, Ryohei eng 2016/07/18 J Chem Ecol. 2016 Jul; 42(7):716-24. doi: 10.1007/s10886-016-0726-7. Epub 2016 Jul 16"