| Title: | Improvement of a headspace solid phase microextraction-gas chromatography/mass spectrometry method for the analysis of wheat bread volatile compounds |
| Author(s): | Raffo A; Carcea M; Castagna C; Magri A; |
| Address: | "Council for Agricultural Research and Economics, Research Centre on Food and Nutrition (CRA-NUT), Via Ardeatina, 546-00178 Rome, Italy. Electronic address: antonio.raffo@entecra.it. Council for Agricultural Research and Economics, Research Centre on Food and Nutrition (CRA-NUT), Via Ardeatina, 546-00178 Rome, Italy. University of Rome 'Sapienza', Department of Chemistry, P. le Aldo Moro, 5-00185 Rome, Italy" |
| DOI: | 10.1016/j.chroma.2015.06.009 |
| ISSN/ISBN: | 1873-3778 (Electronic) 0021-9673 (Linking) |
| Abstract: | "An improved method based on headspace solid phase microextraction combined with gas chromatography-mass spectrometry (HS-SPME/GC-MS) was proposed for the semi-quantitative determination of wheat bread volatile compounds isolated from both whole slice and crust samples. A DVB/CAR/PDMS fibre was used to extract volatiles from the headspace of a bread powdered sample dispersed in a sodium chloride (20%) aqueous solution and kept for 60min at 50 degrees C under controlled stirring. Thirty-nine out of all the extracted volatiles were fully identified, whereas for 95 other volatiles a tentative identification was proposed, to give a complete as possible profile of wheat bread volatile compounds. The use of an array of ten structurally and physicochemically similar internal standards allowed to markedly improve method precision with respect to previous HS-SPME/GC-MS methods for bread volatiles. Good linearity of the method was verified for a selection of volatiles from several chemical groups by calibration with matrix-matched extraction solutions. This simple, rapid, precise and sensitive method could represent a valuable tool to obtain semi-quantitative information when investigating the influence of technological factors on volatiles formation in wheat bread and other bakery products" |
| Keywords: | Bread/*analysis Calibration Dimethylpolysiloxanes/chemistry Food Technology/instrumentation/*methods *Gas Chromatography-Mass Spectrometry Organic Chemicals/*analysis Polyvinyls/chemistry Sodium Chloride/chemistry *Solid Phase Microextraction Triticum/che; |
| Notes: | "MedlineRaffo, Antonio Carcea, Marina Castagna, Claudia Magri, Andrea eng Research Support, Non-U.S. Gov't Netherlands 2015/06/30 J Chromatogr A. 2015 Aug 7; 1406:266-78. doi: 10.1016/j.chroma.2015.06.009. Epub 2015 Jun 14" |
