| Title: | Cyclic AMP mediation of the hormonal stimulation of 14C-acetate incorporation by Heliothis armigera pheromone glands in vitro |
| Address: | "Department of Entomology, Faculty of Agriculture, Rehovot, Israel" |
| DOI: | 10.1016/0303-7207(89)90163-9 |
| ISSN/ISBN: | 0303-7207 (Print) 0303-7207 (Linking) |
| Abstract: | "Evidence is presented for hormone-controlled adenosine 3',5'-cyclic monophosphate (cAMP)-mediated incorporation of 14C-acetate by in vitro pheromone gland preparations of Heliothis armigera. Basal intracellular cAMP concentrations of in vitro pheromone glands increase 28-fold in the presence of methanolic brain-complex extracts after 5 min of incubation. This intracellular cAMP increase shows a dose-dependent response. Studies on isolated pheromone gland preparations show that 8-bromo-cAMP added to the incubation media stimulates acetate incorporation thereby mimicking the hormonal response. This effect was duplicated by forskolin and isobutyl-methylxanthine. In addition, forskolin exhibits a dose-dependent stimulatory effect on the incorporation of 14C-acetate by such isolated in vitro pheromone glands. High-pressure liquid chromatographic analysis of the radioactive products, extracted by hexane from pheromone glands and media, revealed a stimulation by forskolin of a product exhibiting the same retention time as (Z)-11-hexadecenal, the main pheromone component of H. armigera. In addition, thin-layer chromatographic analysis revealed co-elution with (Z)-11-hexadecenal on stimulation by 8-bromo-cAMP" |
| Keywords: | "1-Methyl-3-isobutylxanthine/pharmacology 8-Bromo Cyclic Adenosine Monophosphate/pharmacology Acetates/metabolism Aldehydes/metabolism Animals Brain/physiology Chromatography, High Pressure Liquid Chromatography, Thin Layer Colforsin/pharmacology Cyclic AM;" |
| Notes: | "MedlineRafaeli, A Soroker, V eng Research Support, Non-U.S. Gov't Ireland 1989/08/01 Mol Cell Endocrinol. 1989 Aug; 65(1-2):43-8. doi: 10.1016/0303-7207(89)90163-9" |
