| Title: | Comparative study of the ditrophic interaction between Beauveria bassiana and Plutella xylostella |
| Author(s): | Nithya PR; Manimegalai S; Nakkeeran S; Mohankumar S; |
| Address: | "Division of Agricultural Entomology, Regional Agricultural Research Station, Kerala Agricultural University, Palakkad, Kerala India. GRID: grid.459442.a. ISNI: 0000 0001 2164 6327 Department of Agricultural Entomology, Tamil Nadu Agricultural University, Coimbatore, India. GRID: grid.412906.8. ISNI: 0000 0001 2155 9899 Centre for Plant Molecular Biology and Biotechnology, Tamil Nadu Agricultural University, Coimbatore, India. GRID: grid.412906.8. ISNI: 0000 0001 2155 9899" |
| DOI: | 10.1007/s13205-021-02760-5 |
| ISSN/ISBN: | 2190-572X (Print) 2190-5738 (Electronic) 2190-5738 (Linking) |
| Abstract: | "In the present investigation, hyperparasitic interaction between B. bassiana, TM (MH590235) and P. xylostella was studied through scanning electron microscopy and chromatographic techniques. Dose-mortality responses showed an increase in mortality of larva with an increase in spore concentration. The LC(50) value for B. bassiana isolate TM (MH590235) was 2.4 x 10(7) spores mL(-1). The ditrophic interaction between B. bassiana and P. xylostella after 24 h revealed the adherence of conidia on stemmata, sensory setae, maxillary palpi and legs. After 48 h post-infection (hpi), germination of the conidia and appressorium formation was observed. Formation of hyphae and initiation of fungal networking was observed at 72 hpi. Complete ramification by mycelia and conidiogenesis of B. bassiana was observed over the mycosed cadaver after 168 hpi. Subsequently, typical sympodial conidiophores of B. bassiana bearing secondary spores were also observed. The metabolome profile of healthy larvae of P. xylostella revealed the presence of non-volatile organic compounds (NVOCs) like docosene, nonadecene, palmitic acid and heneicosane. However, the NVOC profile was different in the P. xylostella larvae hyperparasitized by B. bassiana. The metabolites present in the infected cadaver were phthalate esters, hydroxyquebrachamine and lactones" |
| Keywords: | Diamondback moth Entomopathogenic fungi Infection process Metabolomic analysis Scanning electron microscopy; |
| Notes: | "PubMed-not-MEDLINENithya, P R Manimegalai, S Nakkeeran, S Mohankumar, S eng Germany 2021/05/11 3 Biotech. 2021 May; 11(5):223. doi: 10.1007/s13205-021-02760-5. Epub 2021 Apr 18" |
