| Title: | In Vitro Cultures and Volatile Organic Compound Production in Chiliadenus montanus (Vhal.) Brullo |
| Author(s): | Abu-Darwish D; Shibli R; Al-Abdallat AM; |
| Address: | "Department of Horticulture and Crop Science, School of Agriculture, The University of Jordan, Amman 11942, Jordan. Department of Agricultural Biotechnology and Genetic Engineering, Faculty of Agriculture Technology, Al-Ahliyya Amman University, Amman 19328, Jordan" |
| ISSN/ISBN: | 2223-7747 (Print) 2223-7747 (Electronic) 2223-7747 (Linking) |
| Abstract: | "Callus and microshoot cultures were established for Chiliadenus montanus (Vhal.) Brullo. (Asteraceae), a medicinal plant known for producing volatile organic compounds (VOCs). Callus induction was achieved successfully by culturing leaf explants on full-strength Murashige and Skoog medium (MS) supplemented with 2.2 microM 2, 4-dichlorophenoxy acetic acid (2,4-D) and 6.9 microM kinetin (Kin). Successful direct shoot regeneration was achieved using nodal explants cultured onto half-strength MS media supplemented with 1.4 muM Gibberellic Acid (GA(3)) and 4.4 muM 6-Benzylaminopurine (BAP). Indirect microshoots were successfully regenerated using callus cultured on MS media supplemented with 8.8 muM BAP, 2.2 muM Zeatin, and 1.4 muM GA(3) followed by culturing on MS media supplemented with 8.8 muM BAP and 0.5 muM naphthalene acetic acid (NAA). Using wild plant aerial parts, callus and microshoots samples, VOCs were extracted successfully using Headspace Solid-Phase Micro-Extraction (HS-SPME) and analyzed by gas chromatography-mass spectrometry (GC-MS). In wild plant extracts, sesquiterpene hydrocarbons were found to be predominant with the following principal components: Alloaromadendrene (11.92%), trans-Cadina-1(6),4-diene (7.54%), and alpha-caryophyllene (6.77%). The analysis of in vitro microshoots revealed high levels of oxygenated monoterpenes with cis-Myrtanol (16.62%), and beta-Cyclocitral (14.3%) as the main components. Callus extract was dominated by monoterpene hydrocarbons and the main compounds identified were (Z)-beta-Ocimene (22.27%), p-Cymene (15.13%), and alpha-pinene (13.78%). In conclusion, an efficient in vitro production system of VOCs in C. montanus was established that can be used in the future for boosting their production without endangering wild plants" |
| Keywords: | astreraceae callus headspace solid-phase micro-extraction micropropagation microshoots secondary metabolites; |
| Notes: | "PubMed-not-MEDLINEAbu-Darwish, Doaa Shibli, Rida Al-Abdallat, Ayed M eng 19-2020-99/University of Jordan/ Switzerland 2022/05/29 Plants (Basel). 2022 May 17; 11(10):1326. doi: 10.3390/plants11101326" |
