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Enzyme Microb Technol

Title:		Alternative secretory signal sequences for recombinant protein production in Pichia pastoris
Author(s):		Karaoglan M;
Address:		"Department of Food Engineering, Erzincan Binali Yildirim University, Erzincan, Turkiye. Electronic address: mert.karaoglan@erzincan.edu.tr"
Journal Title:		Enzyme Microb Technol
Year:		2023
Volume:		20230513
Issue:
Page Number:		110256 -
DOI:		10.1016/j.enzmictec.2023.110256
ISSN/ISBN:		1879-0909 (Electronic) 0141-0229 (Linking)
Abstract:		"Extracellular protein production is primarily preferred to facilitate the downstream processes in recombinant protein production. Secretion of recombinant proteins is mediated by the processing of signal peptides in their N-terminal portion by the secretory mechanism of host expression systems. These molecular elements involved in secretion are functionally interchangeable between different species and secretion sequence screening is one of the widely used approaches to improve extracellular protein production. In this study, alpha-mating and protein internal repeats (PIR) secretion sequences isolated from different yeasts (Kluyveromyces lactis, Kluyveromyces marxianus and Hansenula polymorpha) were tested in Pichia pastoris for the production of two different proteins (alpha-amylase and xylanase) and compared with the well-known secretory signals, S. cerevisiae alpha-mating factor (Sc-MF) and P. pastoris protein internal repeats PIR (PpPIR). The results obtained showed the potential of signal sequences tested. Among the tested peptides, the highest yields were achieved with H. polymorpha protein internal repeats (HpPIR) and K. lactis alpha-mating factor (Kl-MF) for xylanase and K. marxianus protein internal repeats (KmPIR) and K. lactis alpha-mating factor (Kl-MF) for amylase. In further studies, these sequences can be evaluated as alternatives in the production of different proteins in P. pastoris and in the production of recombinant proteins in different expression systems"
Keywords:		*Protein Sorting Signals/genetics *Saccharomyces cerevisiae/metabolism Mating Factor/genetics/metabolism Pichia/genetics/metabolism Recombinant Proteins/metabolism Enhancing protein secretion Extracellular protein production Pichia pastoris Yeast secretor;
Notes:		"MedlineKaraoglan, Mert eng 2023/05/18 Enzyme Microb Technol. 2023 Aug; 168:110256. doi: 10.1016/j.enzmictec.2023.110256. Epub 2023 May 13"