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Methods Mol Biol

Title:		Cell fusion assays for yeast mating pairs
Author(s):		Grote E;
Address:		"Department of Biochemistry and Molecular Biology, Johns Hopkins Bloomberg School of Public Health, Baltimore, MD, USA"
Journal Title:		Methods Mol Biol
Year:		2008
Volume:		475
Issue:
Page Number:		165 - 196
DOI:		10.1007/978-1-59745-250-2_10
ISSN/ISBN:		1064-3745 (Print) 1064-3745 (Linking)
Abstract:		"Yeast mating provides an accessible genetic system for the discovery of fundamental mechanisms in eukaryotic cell fusion. Although aspects of yeast mating related to pheromone signaling and polarized growth have been intensively investigated, fusion itself is poorly understood. This chapter describes methods for measuring the overall efficiency of yeast cell fusion and for monitoring various stages of the fusion process including cell wall remodeling, plasma membrane fusion, and nuclear fusion"
Keywords:		Cytological Techniques/*methods Genetic Complementation Test Luminescent Proteins/metabolism Pyridinium Compounds/metabolism Quaternary Ammonium Compounds/metabolism Reproduction Saccharomyces cerevisiae/*cytology/growth & development/*physiology Vacuoles;
Notes:		"MedlineGrote, Eric eng Research Support, Non-U.S. Gov't 2008/11/04 Methods Mol Biol. 2008; 475:165-96. doi: 10.1007/978-1-59745-250-2_10"