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J Chem Ecol

Title:		Changes in rice allelopathy and rhizosphere microflora by inhibiting rice phenylalanine ammonia-lyase gene expression
Author(s):		Fang C; Zhuang Y; Xu T; Li Y; Li Y; Lin W;
Address:		"Key Laboratory of Biopesticide and Chemical Biology, Ministry of Education, Fuzhou 350002, People's Republic of China"
Journal Title:		J Chem Ecol
Year:		2013
Volume:		20130206
Issue:		2
Page Number:		204 - 212
DOI:		10.1007/s10886-013-0249-4
ISSN/ISBN:		1573-1561 (Electronic) 0098-0331 (Linking)
Abstract:		"Gene expression of phenylalanine ammonia-lyase (PAL) in allelopathic rice PI312777 was inhibited by RNA interference (RNAi). Transgenic rice showed lower levels of PAL gene expression and PAL activity than wild type rice (WT). The concentrations of phenolic compounds were lower in the root tissues and root exudates of transgenic rice than in those of wild type plants. When barndyardgrass (BYG) was used as the receiver plant, the allelopathic potential of transgenic rice was reduced. The sizes of the bacterial and fungal populations in rice rhizospheric soil at the 3-, 5-, and 7-leaf stages were estimated by using quantitative PCR (qPCR), which showed a decrease in both populations at all stages of leaf development analyzed. However, PI312777 had a larger microbial population than transgenic rice. In addition, in T-RFLP studies, 14 different groups of bacteria were detected in WT and only 6 were detected in transgenic rice. This indicates that there was less rhizospheric bacterial diversity associated with transgenic rice than with WT. These findings collectively suggest that PAL functions as a positive regulator of rice allelopathic potential"
Keywords:		"Echinochloa/growth & development Gene Expression Regulation, Plant Oryza/*genetics/*metabolism Phenols/metabolism Phenylalanine Ammonia-Lyase/*genetics Pheromones/*metabolism Plant Roots/genetics/*metabolism Plants, Genetically Modified/genetics/metabolis;"
Notes:		"MedlineFang, Changxun Zhuang, Yuee Xu, Tiecheng Li, Yingzhe Li, Yue Lin, Wenxiong eng Research Support, Non-U.S. Gov't 2013/02/07 J Chem Ecol. 2013 Feb; 39(2):204-12. doi: 10.1007/s10886-013-0249-4. Epub 2013 Feb 6"