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J Hazard Mater

Title:		Pheromone effect of estradiol regulates the conjugative transfer of pCF10 carrying antibiotic resistance genes
Author(s):		Yang X; Niu Y; Yang Y; Zhou H; Li J; Fu X; Shen Z; Wang J; Qiu Z;
Address:		"Tianjin Institute of Environmental and Operational Medicine, Tianjin 300050, China. Tianjin Institute of Environmental and Operational Medicine, Tianjin 300050, China; Shanghai Ocean University, Shanghai 201306, China. Tianjin Institute of Environmental and Operational Medicine, Tianjin 300050, China; Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China. Tianjin Institute of Environmental and Operational Medicine, Tianjin 300050, China. Electronic address: jingfengwang@hotmail.com. Tianjin Institute of Environmental and Operational Medicine, Tianjin 300050, China. Electronic address: zhigangqiu99@gmail.com"
Journal Title:		J Hazard Mater
Year:		2023
Volume:		20230224
Issue:
Page Number:		131087 -
DOI:		10.1016/j.jhazmat.2023.131087
ISSN/ISBN:		1873-3336 (Electronic) 0304-3894 (Linking)
Abstract:		"Horizontal gene transfer (HGT) mediated by conjugative plasmids greatly contributes to bacteria evolution and the transmission of antibiotic resistance genes (ARGs). In addition to the selective pressure imposed by extensive antibiotic use, environmental chemical pollutants facilitate the dissemination of antibiotic resistance, consequently posing a serious threat to the ecological environment. Presently, the majority of studies focus on the effects of environmental compounds on R plasmid-mediated conjugation transfer, and pheromone-inducible conjugation has largely been neglected. In this study, we explored the pheromone effect and potential molecular mechanisms of estradiol in promoting the conjugative transfer of pCF10 plasmid in Enterococcus faecalis. Environmentally relevant concentrations of estradiol significantly increased the conjugative transfer of pCF10 with a maximum frequency of 3.2 x 10(-2), up to 3.5-fold change compared to that of control. Exposure to estradiol induced the activation of pheromone signaling cascade by increasing the expression of ccfA. Furthermore, estradiol might directly bind to the pheromone receptor PrgZ and promote pCF10 induction and finally enhance the conjugative transfer of pCF10. These findings cast valuable insights on the roles of estradiol and its homolog in increasing antibiotic resistance and the potential ecological risk"
Keywords:		"*Anti-Bacterial Agents/metabolism *Pheromones/pharmacology/genetics/metabolism Estradiol/pharmacology/metabolism Plasmids/genetics Drug Resistance, Microbial/genetics Enterococcus faecalis/genetics/metabolism Gene Transfer, Horizontal Antibiotic resistanc;"
Notes:		"MedlineYang, Xiaobo Niu, Yuanyuan Yang, Yutong Zhou, Hongrui Li, Jing Fu, Xinyue Shen, Zhiqiang Wang, Jingfeng Qiu, Zhigang eng Research Support, Non-U.S. Gov't Netherlands 2023/03/09 J Hazard Mater. 2023 Jun 5; 451:131087. doi: 10.1016/j.jhazmat.2023.131087. Epub 2023 Feb 24"