| Title: | Urinary Proteins of Female Domestic Dog (Canis familiaris) during Ovarian Cycle |
| Author(s): | Woszczylo M; Pasikowski P; Devaraj S; Kokocinska A; Szumny A; Skwark MJ; Nizanski W; Dzieciol M; |
| Address: | "Department of Reproduction and Clinic of Farm Animals, Wroclaw University of Environmental and Life Sciences, Plac Grunwaldzki 49, 50-366 Wroclaw, Poland. Captor Therapeutics Inc., Dunska 11, 54-427 Wroclaw, Poland. Department of Biotechnology, School of BioSciences and Technology, Vellore Institute of Technology, Vellore 632014, India. Institute of Biological Bases of Animal Production, University of Life Sciences in Lublin, 13 Akademicka St., 20-950 Lublin, Poland. Department of Chemistry, Wroclaw University of Environmental and Life Sciences, C.K. Norwida 25, 50-375 Wroclaw, Poland. InstaDeep Ltd., 5 Merchant Square, London W2 1AY, UK" |
| ISSN/ISBN: | 2306-7381 (Electronic) 2306-7381 (Linking) |
| Abstract: | "The presence and identity of non-volatile chemical signals remain elusive in canines. In this study, we aim to evaluate the urinary proteins of female domestic dogs in the estrus and anestrus phases to evidence the presence of non-volatile chemical signals and to elucidate their identities. We collected urine samples from eight female dogs in the estrus and anestrus phases. A total of 240 proteins were identified in the urine samples using liquid chromatography-mass spectrometry (LC-MS analysis). The comparison of the proteins revealed a significant difference between the estrus and anestrus urine. We identified proteins belonging to the lipocalin family of canines (beta-lactoglobulin-1 and beta-lactoglobulin-2, P33685 and P33686, respectively), one of whose function was the transport of pheromones and which was present only in the estrus urine samples. Moreover, proteins such as Clusterin (CLU), Liver-expressed antimicrobial peptide 2 (LEAP2), and Proenkephalin (PENK) were more abundant in the estrus urine when compared to the anestrus urine. LEAP2 was recently described as a ghrelin receptor antagonist and implicated in regulating food intake and body weight in humans and mice. Proenkephalin, a polypeptide hormone cleaved into opioid peptides, was also recognized as a candidate to determine kidney function. As of yet, none of these have played a role in chemical communication. Clusterin, an extracellular chaperone protecting from protein aggregation implicated in stress-induced cell apoptosis, is a plausible candidate in chemical communication, which is a claim that needs to be ascertained further. Data are available via ProteomeXchange with the identifier PXD040418" |
| Keywords: | Animals;Lc-ms/ms chemical signals dogs estrus proteome urine; |
| Notes: | "PubMed-not-MEDLINEWoszczylo, Martyna Pasikowski, Pawel Devaraj, Sankarganesh Kokocinska, Agata Szumny, Antoni Skwark, Marcin J Nizanski, Wojciech Dzieciol, Michal eng UMO-2015/17/B/NZ8/02411./The National Science Centre (Poland)/ APC/BPC/Wroclaw University of Environmental and Life Sciences and the University of Life Sciences/ APC/BPC/Life Sciences and the University of Life Sciences, Lublin/ Switzerland 2023/04/27 Vet Sci. 2023 Apr 14; 10(4):292. doi: 10.3390/vetsci10040292" |
