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J Chem Ecol

Title:		The preferential binding of a sensory organ specific odorant binding protein of the alfalfa plant bug Adelphocoris lineolatus AlinOBP10 to biologically active host plant volatiles
Author(s):		Sun L; Gu SH; Xiao HJ; Zhou JJ; Guo YY; Liu ZW; Zhang YJ;
Address:		"Key Laboratory of Integrated Management of Crop Diseases and Pests (Ministry of Education), College of Plant Protection, Nanjing Agricultural University, Nanjing, 210095, China"
Journal Title:		J Chem Ecol
Year:		2013
Volume:		20130817
Issue:		9
Page Number:		1221 - 1231
DOI:		10.1007/s10886-013-0333-9
ISSN/ISBN:		1573-1561 (Electronic) 0098-0331 (Linking)
Abstract:		"Semiochemicals such as sex pheromones and plant volatiles are crucial components of insect mating systems and host plant localization. In the olfactory signal transduction pathway, odorant-binding proteins (OBPs) are important elements that function in the first step of the pathway by carrying hydrophobic semiochemicals across the sensillum lymph to the olfactory receptors (ORs). In this study, we examined the binding affinities of semiochemicals to AlinOBP10, a putative OBP from the alfalfa plant bug, Adelphocoris lineolatus, that we demonstrate is expressed mainly in sensory organs. We then characterized the biological activities of the high affinity semiochemicals by measuring their electrophysiological activities in antennae and behavioral responses in the plant bug. AlinOBP10 displayed weak binding affinities to two major putative pheromone components, hexyl butyrate and (E)-2-hexenyl butyrate. In contrast, AlinOBP10 exhibited higher binding affinities to six host plant volatiles, namely myrcene, beta-pinene, beta-ionone, 3-hexanone, (E)-2-hexenal, and 1-hexanol. The biological activities of these six putative ligands were further studied in electroantennogram recordings and Y-tube olfactometer trials. The three compounds, (E)-2-hexenal, 1-hexanol, and 3-hexanone elicited strong electrophysiological responses, but elicited distinct behaviors. While 3-hexanone was attractive to female adults, (E)-2-hexenal and 1-hexanol were significant repellents. Although a weak electrophysiological response was elicited with beta-pinene, it was a strong repellent. These results demonstrate that AlinOBP10 can interact with attractants, as well as repellents, with some specificity toward plant volatiles over sex pheromones"
Keywords:		"Animals Arthropod Antennae/metabolism Behavior, Animal/physiology Female Heteroptera/*physiology Male Pheromones/*metabolism Receptors, Odorant/*metabolism Recombinant Proteins/metabolism Volatile Organic Compounds/metabolism;"
Notes:		"MedlineSun, Liang Gu, Shao-Hua Xiao, Hai-Jun Zhou, Jing-Jiang Guo, Yu-Yuan Liu, Ze-Wen Zhang, Yong-Jun eng Research Support, Non-U.S. Gov't 2013/08/21 J Chem Ecol. 2013 Sep; 39(9):1221-31. doi: 10.1007/s10886-013-0333-9. Epub 2013 Aug 17"