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New Phytol

Title:		The root-knot nematode effector MiMSP32 targets host 12-oxophytodienoate reductase 2 to regulate plant susceptibility
Author(s):		Verhoeven A; Finkers-Tomczak A; Prins P; Valkenburg-van Raaij DR; van Schaik CC; Overmars H; van Steenbrugge JJM; Tacken W; Varossieau K; Slootweg EJ; Kappers IF; Quentin M; Goverse A; Sterken MG; Smant G;
Address:		"Laboratory of Nematology, Department of Plant Sciences, Wageningen University & Research, Droevendaalsesteeg 1, 6708 PB, Wageningen, the Netherlands. Plant Stress Resilience, Utrecht University, Padualaan 8, 3584 CH, Utrecht, the Netherlands. Plant-Environment Signaling, Utrecht University, Padualaan 8, 3584 CH, Utrecht, the Netherlands. Laboratory of Plant Physiology, Department of Plant Sciences, Wageningen University & Research, Droevendaalsesteeg 1, 6708 PB, Wageningen, the Netherlands. INRAE, Universite Cote d'Azur, CNRS, ISA, F-06903, Sophia Antipolis, France"
Journal Title:		New Phytol
Year:		2023
Volume:		20230131
Issue:		6
Page Number:		2360 - 2374
DOI:		10.1111/nph.18653
ISSN/ISBN:		1469-8137 (Electronic) 0028-646X (Linking)
Abstract:		"To establish persistent infections in host plants, herbivorous invaders, such as root-knot nematodes, must rely on effectors for suppressing damage-induced jasmonate-dependent host defenses. However, at present, the effector mechanisms targeting the biosynthesis of biologically active jasmonates to avoid adverse host responses are unknown. Using yeast two-hybrid, in planta co-immunoprecipitation, and mutant analyses, we identified 12-oxophytodienoate reductase 2 (OPR2) as an important host target of the stylet-secreted effector MiMSP32 of the root-knot nematode Meloidogyne incognita. MiMSP32 has no informative sequence similarities with other functionally annotated genes but was selected for the discovery of novel effector mechanisms based on evidence of positive, diversifying selection. OPR2 catalyzes the conversion of a derivative of 12-oxophytodienoate to jasmonic acid (JA) and operates parallel to 12-oxophytodienoate reductase 3 (OPR3), which controls the main pathway in the biosynthesis of jasmonates. We show that MiMSP32 targets OPR2 to promote parasitism of M. incognita in host plants independent of OPR3-mediated JA biosynthesis. Artificially manipulating the conversion of the 12-oxophytodienoate by OPRs increases susceptibility to multiple unrelated plant invaders. Our study is the first to shed light on a novel effector mechanism targeting this process to regulate the susceptibility of host plants"
Keywords:		Animals *Oxidoreductases Acting on CH-CH Group Donors/metabolism Oxidoreductases/metabolism Biological Transport *Tylenchoidea/physiology Plant Diseases Meloidogyne incognita 12-oxophytodienoic acid effector host target jasmonates nematode positive select;
Notes:		"MedlineVerhoeven, Ava Finkers-Tomczak, Anna Prins, Pjotr Valkenburg-van Raaij, Debbie R van Schaik, Casper C Overmars, Hein van Steenbrugge, Joris J M Tacken, Wannes Varossieau, Koen Slootweg, Erik J Kappers, Iris F Quentin, Michael Goverse, Aska Sterken, Mark G Smant, Geert eng Research Support, Non-U.S. Gov't England 2022/12/03 New Phytol. 2023 Mar; 237(6):2360-2374. doi: 10.1111/nph.18653. Epub 2023 Jan 31"