| Title: | Volatile Organic Compound from Trichoderma asperelloides TSU1: Impact on Plant Pathogenic Fungi |
| Author(s): | Ruangwong OU; Wonglom P; Suwannarach N; Kumla J; Thaochan N; Chomnunti P; Pitija K; Sunpapao A; |
| Address: | "Department of Entomology and Plant Pathology, Faculty of Agriculture, Chiang Mai University, Chiang Mai 50200, Thailand. Innovative Agriculture Research Center, Faculty of Agriculture, Chiang Mai University, Chiang Mai 50200, Thailand. Faculty of Technology and Community Development, Phatthalung Campus, Thaksin University, 222 Moo 2, Ban Phrao Sub-District, Pa Payom District, Phattalung 93110, Thailand. Research Center of Microbial Diversity and Sustainable Utilization, Chiang Mai University, Chiang Mai 50200, Thailand. Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. Agricultural Innovation and Management Division (Pest Management), Faculty of Natural Resources, Prince of Songkla University, Hatyai, Songkhla 90110, Thailand. School of Science, Mae Fah Luang University, Chiang Rai 57100, Thailand. Perkin Elmer Co. Ltd., 290 Soi 17, Rama 9 Rd., Bangkapi, Huay Kwang, Bangkok 10310, Thailand" |
| ISSN/ISBN: | 2309-608X (Electronic) 2309-608X (Linking) |
| Abstract: | "Soil microorganisms are well studied for their beneficial effects on plant growth and their impact on biocontrol agents. The production of volatile antifungal compounds emitted from soil fungi is considered to be an effective ability that can be applied in biofumigants in the control of plant diseases. A soil fungus, Trichoderma asperelloides TSU1, was isolated from flamingo flower cultivated soil and identified on the basis of the morphology and molecular analysis of the internal transcribed spacer (ITS), rpb2, and tef1-alpha genes. To test T. asperelloides TSU1-produced volatile organic compounds (VOCs) with antifungal activity, the sealed plate method was used. The VOCs of T. asperelloides TSU1 inhibited the mycelial growth of fungal pathogens that were recently reported as emerging diseases in Thailand, namely, Corynespora cassiicola, Fusarium incarnatum, Neopestalotiopsis clavispora, N. cubana, and Sclerotium rolfsii, with a percentage inhibition range of 38.88-68.33%. Solid-phase microextraction (SPME) was applied to trap VOCs from T. asperelloides TSU1 and tentatively identify them through gas chromatography-mass spectrometry (GC/MS). A total of 17 compounds were detected in the VOCs of T. asperelloides TSU1, and the dominant compounds were identified as fluoro(trinitro)methane (18.192% peak area) and 2-phenylethanol (9.803% peak area). Interestingly, the commercial 2-phenyethanol showed antifungal activity against fungal pathogens that were similar to the VOCs of T. asperelloides TSU1 by bioassay. On the basis of our study's results, T. asperelloides TSU1 isolated from soil displayed antifungal abilities via the production of VOCs responsible for restricting pathogen growth" |
| Keywords: | Trichoderma VOCs antifungal activity plant pathogen soil fungi; |
| Notes: | "PubMed-not-MEDLINERuangwong, On-Uma Wonglom, Prisana Suwannarach, Nakarin Kumla, Jaturong Thaochan, Narit Chomnunti, Putarak Pitija, Kitsada Sunpapao, Anurag eng NRCT5-RSA63022-01/National Research Council of Thailand/ Switzerland 2021/04/04 J Fungi (Basel). 2021 Mar 5; 7(3):187. doi: 10.3390/jof7030187" |
