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PLoS One

Title:		Transgenic Tobacco Overexpressing Tea cDNA Encoding Dihydroflavonol 4-Reductase and Anthocyanidin Reductase Induces Early Flowering and Provides Biotic Stress Tolerance
Author(s):		Kumar V; Nadda G; Kumar S; Yadav SK;
Address:		"Biotechnology Division, CSIR-Institute of Himalayan Bioresource Technology, Council of Scientific and Industrial Research, Palampur, Himachal Pradesh, India"
Journal Title:		PLoS One
Year:		2013
Volume:		20130618
Issue:		6
Page Number:		e65535 -
DOI:		10.1371/journal.pone.0065535
ISSN/ISBN:		1932-6203 (Electronic) 1932-6203 (Linking)
Abstract:		"Flavan-3-ols contribute significantly to flavonoid content of tea (Camellia sinensis L.). Dihydroflavonol 4-reductase (DFR) and anthocyanidin reductase (ANR) are known to be key regulatory enzymes of flavan-3-ols biosynthesis. In this study, we have generated the transgenic tobacco overexpressing individually tea cDNA CsDFR and CsANR encoding for DFR and ANR to evaluate their influence on developmental and protective abilities of plant against biotic stress. The transgenic lines of CsDFR and CsANR produced early flowering and better seed yield. Both types of transgenic tobacco showed higher content of flavonoids than control. Flavan-3-ols such as catechin, epicatechin and epicatechingallate were found to be increased in transgenic lines. The free radical scavenging activity of CsDFR and CsANR transgenic lines was improved. Oxidative stress was observed to induce lesser cell death in transgenic lines compared to control tobacco plants. Transgenic tobacco overexpressing CsDFR and CsANR also showed resistance against infestation by a tobacco leaf cutworm Spodoptera litura. Results suggested that the overexpression of CsDFR and CsANR cDNA in tobacco has improved flavonoids content and antioxidant potential. These attributes in transgenic tobacco have ultimately improved their growth and development, and biotic stress tolerance"
Keywords:		"*Adaptation, Physiological Alcohol Oxidoreductases/*genetics Animals Anthocyanins/*metabolism DNA, Complementary/*genetics *Flowers Herbivory Oxidative Stress Oxidoreductases/*genetics/metabolism Plants, Genetically Modified/*genetics Spodoptera/physiolog;"
Notes:		"MedlineKumar, Vinay Nadda, Gireesh Kumar, Sanjay Yadav, Sudesh Kumar eng Research Support, Non-U.S. Gov't 2013/07/05 PLoS One. 2013 Jun 18; 8(6):e65535. doi: 10.1371/journal.pone.0065535. Print 2013"