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Int J Mol Sci

Title:		Transcriptome Analyses Reveal the Aroma Terpeniods Biosynthesis Pathways of Primula forbesii Franch. and the Functional Characterization of the PfDXS2 Gene
Author(s):		Jia Y; Yin X; Yang H; Xiang Y; Ding K; Pan Y; Jiang B; Yong X;
Address:		"College of Landscape Architecture, Sichuan Agricultural University, Chengdu 611130, China"
Journal Title:		Int J Mol Sci
Year:		2023
Volume:		20230812
Issue:		16
Page Number:		-
DOI:		10.3390/ijms241612730
ISSN/ISBN:		1422-0067 (Electronic) 1422-0067 (Linking)
Abstract:		"Primula forbesii Franch. is a unique biennial herb with a strong floral fragrance, making it an excellent material for studying the aroma characteristics of the genus Primula. The floral scent is an important ornamental trait that facilitates fertilization. However, the molecular mechanism regulating the floral scent in Primula is unknown. In order to better understand the biological mechanisms of floral scents in this species, this study used RNA sequencing analysis to discuss the first transcriptome sequence of four flowering stages of P. forbesii, which generated 12 P. forbesii cDNA libraries with 79.64 Gb of clean data that formed 51,849 unigenes. Moreover, 53.26% of the unigenes were annotated using public databases. P. forbesii contained 44 candidate genes covering all known enzymatic steps for the biosynthesis of volatile terpenes, the major contributor to the flower's scent. Finally, 1-deoxy-d-xylulose 5-phosphate synthase gene of P. forbesii (PfDXS2, MK370094), the first key enzyme gene in the 2-c-methyl-d-erythritol 4-phosphate (MEP) pathway of terpenoids, was cloned and functionally verified using virus-induced gene silencing (VIGs). The results showed that PfDXS2-silencing significantly reduced the relative concentrations of main volatile terpenes. This report is the first to present molecular data related to aroma metabolites biosynthesis pathways and the functional characterization of any P. forbesii gene. The data on RNA sequencing provide comprehensive information for further analysis of other plants of the genus Primula"
Keywords:		*Odorants *Primula/genetics Gene Expression Profiling Transcriptome Pheromones Dxs Primula RNA-seq VIGs floral scent terpenoids;
Notes:		"MedlineJia, Yin Yin, Xiancai Yang, Hongchen Xiang, Yuanfen Ding, Keying Pan, Yuanzhi Jiang, Beibei Yong, Xue eng 32001356/National Natural Science Foundation of China/ Switzerland 2023/08/26 Int J Mol Sci. 2023 Aug 12; 24(16):12730. doi: 10.3390/ijms241612730"