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Proc Natl Acad Sci U S A

Title:		Dynamic functional evolution of an odorant receptor for sex-steroid-derived odors in primates
Author(s):		Zhuang H; Chien MS; Matsunami H;
Address:		"Department of Molecular Genetics and Microbiology and Neurobiology, Duke University Medical Center, Durham, NC 27710, USA. hanyizhuang@sjtu.edu.cn"
Journal Title:		Proc Natl Acad Sci U S A
Year:		2009
Volume:		20091202
Issue:		50
Page Number:		21247 - 21251
DOI:		10.1073/pnas.0808378106
ISSN/ISBN:		1091-6490 (Electronic) 0027-8424 (Print) 0027-8424 (Linking)
Abstract:		"Odorant receptors are among the fastest evolving genes in animals. However, little is known about the functional changes of individual odorant receptors during evolution. We have recently demonstrated a link between the in vitro function of a human odorant receptor, OR7D4, and in vivo olfactory perception of 2 steroidal ligands--androstenone and androstadienone--chemicals that are shown to affect physiological responses in humans. In this study, we analyzed the in vitro function of OR7D4 in primate evolution. Orthologs of OR7D4 were cloned from different primate species. Ancestral reconstruction allowed us to reconstitute additional putative OR7D4 orthologs in hypothetical ancestral species. Functional analysis of these orthologs showed an extremely diverse range of OR7D4 responses to the ligands in various primate species. Functional analysis of the nonsynonymous changes in the Old World Monkey and Great Ape lineages revealed a number of sites causing increases or decreases in sensitivity. We found that the majority of the functionally important residues in OR7D4 were not predicted by the maximum likelihood analysis detecting positive Darwinian selection"
Keywords:		"Animals Base Sequence Cercopithecidae *Evolution, Molecular Gonadal Steroid Hormones/metabolism/*physiology Hominidae Humans Ligands Likelihood Functions Molecular Sequence Data Primates Receptors, Odorant/genetics/physiology Receptors, Pheromone/*genetic;"
Notes:		"MedlineZhuang, Hanyi Chien, Ming-Shan Matsunami, Hiroaki eng T32 GM007754/GM/NIGMS NIH HHS/ Research Support, N.I.H., Extramural 2009/12/04 Proc Natl Acad Sci U S A. 2009 Dec 15; 106(50):21247-51. doi: 10.1073/pnas.0808378106. Epub 2009 Dec 2"